Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang, 315211, People's Republic of China.
Appl Biochem Biotechnol. 2018 Mar;184(3):1009-1023. doi: 10.1007/s12010-017-2607-0. Epub 2017 Sep 20.
A novel ZnO nanowire/macroporous SiO composite was used as a support to immobilize chloroperoxidase (CPO) by in situ cross-linking method. An anionic bi-epoxy compound was synthesized and used as a long-chained anionic cross-linker, and it was adsorbed on the surface of ZnO nanowires through static interaction before reaction with CPO, creating a new approach to change the structure, property, and catalytic performance of the produced cross-linking enzyme aggregates (CLEAs) of CPO. The immobilized CPO showed high activity in the decolorization of three azo dyes. The effect of various conditions such as the loading amount of CPO, solution pH, temperature, and dye concentration was optimized on the decolorization. Under optimized conditions, the decolorization percentage of Acid Blue 113, Direct Black 38, and Acid Black 10 BX reached as high as 95.4, 92.3, and 89.1%, respectively. The immobilized CPO exhibited much better thermostability and resistance to pH inactivation than free CPO. The storage stability and reusability were greatly improved through the immobilization. It was found from the decolorization of Acid Blue 113 that 83.6% of initial activity retained after incubation at 4 °C for 60 days and that 80.9% of decolorization efficiency retained after 12 cycles of reuses.
一种新型的 ZnO 纳米线/大孔 SiO2复合材料被用作固定化漆酶的载体，通过原位交联法。合成了一种阴离子双环氧化合物作为长链阴离子交联剂，它在与漆酶反应之前通过静电相互作用吸附在 ZnO 纳米线表面，为改变漆酶交联酶聚集体（CLEAs）的结构、性质和催化性能创造了一种新方法。固定化漆酶在三种偶氮染料的脱色中表现出很高的活性。优化了各种条件，如漆酶的负载量、溶液 pH 值、温度和染料浓度对脱色的影响。在优化条件下，酸性蓝 113、直接黑 38 和酸性黑 10 BX 的脱色率分别高达 95.4%、92.3%和 89.1%。固定化漆酶的热稳定性和 pH 失活抗性明显优于游离漆酶。通过固定化，大大提高了其储存稳定性和可重复使用性。从酸性蓝 113 的脱色实验中发现，在 4°C 孵育 60 天后，初始活性保留了 83.6%，在重复使用 12 次后，脱色效率保留了 80.9%。
