Kuang Xue-Jun, Zou Li-Qiu, Li Ying, Sun Chao, Chen Shi-Lin
Institute of Medicinal Plant Development, China Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2016 Nov;41(22):4112-4118. doi: 10.4268/cjcmm20162205.
Natural products with complex and diverse structures are the major sources of new drugs. The biosynthesis of natural products is considered to be one of the best ways to solve the problems of complex and scarce natural products. DNA assembly technology and genome editing technology are two key technologies in the emerging interdisciplinary field of synthetic biology. A number of novel DNA assembly methods developed in the last few years have paved the way for the engineering of high molecular weight DNA molecules, including whole genomes, hence, it can realize the reconstruction of the metabolic pathways and speed up optimization process. A wide variety of new tools for microbial genome editing will be applied widely to modify the chassis genome to increase its adaptation with the exogenetic pathways. This article summarized the latest advance with respect to DNA assembly and genome editing, which aims to provide help for reconstruction and optimization of the synthetic biological systems of natural products.
结构复杂多样的天然产物是新药的主要来源。天然产物的生物合成被认为是解决天然产物结构复杂且稀缺问题的最佳途径之一。DNA组装技术和基因组编辑技术是新兴的合成生物学交叉领域中的两项关键技术。过去几年开发的一些新型DNA组装方法为包括全基因组在内的高分子量DNA分子的工程改造铺平了道路，因此，它可以实现代谢途径的重建并加速优化过程。各种各样的微生物基因组编辑新工具将被广泛应用于修饰底盘基因组，以增强其对外源途径的适应性。本文总结了DNA组装和基因组编辑方面的最新进展，旨在为天然产物合成生物学系统的重建和优化提供帮助。
