Gao Fen, Hao Rui, Qin Xue-Mei, Lei Zhen-Hong, Wang Yu-Long, Wang Meng-Liang
Institute of Applied Chemistry, Shanxi University, Taiyuan 030006, China.
Institute of Biotechnology, Shanxi University, Taiyuan 030006, China.
Zhongguo Zhong Yao Za Zhi. 2016 Nov;41(22):4188-4193. doi: 10.4268/cjcmm20162217.
The antagonistic effect of Bacillus spp. against Fusarium solani was evaluated by living body dual culture and Oxford cup method. The plant growth promoting properties of those strains that had obvious and stable antifungal activity were then tested. The results showed that the living body and bacteria-free fermentation filtrate of strain G10 both had obvious and stable antifungal effect to F. solani. Besides, the strain possessed such growth promoting properties as phosphate solubilization, nitrogen fixation, and production of IAA, amylase and HCN. Strain G10 was classified and identified as B. subtilis by a combination of morphological, physiological and biochemical tests, 16 SrDNA gene sequence analysis and the BBL CrystalTM bacteria identification. In conclusion, B. subtilis G10 has the basic characteristics of multifunctional strains and could be one of the microbiological resources for developing special bio-control agent against Astragalus root rot.
通过活体双重培养和牛津杯法评估了芽孢杆菌属对茄类镰刀菌的拮抗作用。然后对那些具有明显且稳定抗真菌活性的菌株的促植物生长特性进行了测试。结果表明，菌株G10的活体和无菌发酵滤液对茄类镰刀菌均具有明显且稳定的抗真菌作用。此外，该菌株具有溶解磷酸盐、固氮以及产生吲哚乙酸、淀粉酶和HCN等促生长特性。通过形态学、生理生化测试、16 SrDNA基因序列分析以及BBL CrystalTM细菌鉴定相结合的方法，将菌株G10分类鉴定为枯草芽孢杆菌。综上所述，枯草芽孢杆菌G10具有多功能菌株的基本特征，可作为开发防治黄芪根腐病专用生物防治剂的微生物资源之一。
