Wroblewski Danielle, Cole Jocelyn, McGinnis Jana, Perez Maria, Wilson Harriet, Mingle Lisa A, Musser Kimberlee A, Wolfgang William J
Wadsworth Center, Bacteriology Laboratory, New York State Department of Health, PO Box 22002, Albany, NY 12201, USA.
Present address: Wadsworth Center, Virology Laboratory, New York State Department of Health, PO Box 22002, Albany, NY 12201, USA.
Int J Syst Evol Microbiol. 2017 Nov;67(11):4304-4310. doi: 10.1099/ijsem.0.002148. Epub 2017 Sep 21.
Three independent isolates of Gram-reaction-negative cocci collected from two New York State patients and a dog's mouth in California were subjected to a polyphasic analysis. The 16S rRNA gene sequence similarity among these isolates is 99.66 to 99.86 %. The closest species with a validly published name is Neisseria zoodegmatis (98.7 % 16S rRNA gene sequence similarity) with six additional species of the genus Neisseria with greater than 97 % similarity. Average nucleotide identity (ANI) and genome-to-genome distance calculator (GGDC 2.0) analysis on whole genome sequence data support the three novel isolates as being from a single species that is distinct from all other closely related species of the genus Neisseria. Phylogenetic analysis of 16S rRNA gene sequences and ribosomal multilocus sequence typing (rMLST) indicate the novel species belongs in the genus Neisseria. This assignment is further supported by the predominant cellular fatty acids composition of C16 : 0, summed feature 3 (C16 : 1ω7c/C15 : 0iso 2-OH), and C18 : 1ω7c, and phenotypic characters. The name Neisseria dumasiana sp. nov. is proposed, and the type strain is 93087 (=DSM 104677=LMG 30012 ).
从纽约州的两名患者以及加利福尼亚州一只狗的口腔中分离出的三株革兰氏阴性球菌独立菌株，进行了多相分析。这些菌株之间的16S rRNA基因序列相似性为99.66%至99.86%。已有效发表名称的最相近物种是动物咬奈瑟菌（16S rRNA基因序列相似性为98.7%），奈瑟菌属还有另外六个物种的相似性大于97%。对全基因组序列数据进行的平均核苷酸同一性（ANI）和基因组到基因组距离计算器（GGDC 2.0）分析支持这三株新分离菌株属于一个与奈瑟菌属所有其他密切相关物种不同的单一物种。16S rRNA基因序列的系统发育分析和核糖体多位点序列分型（rMLST）表明该新物种属于奈瑟菌属。C16 : 0、总和特征3（C16 : 1ω7c/C15 : 0iso 2-OH）和C18 : 1ω7c的主要细胞脂肪酸组成以及表型特征进一步支持了这一分类。提议将其命名为杜马斯奈瑟菌（Neisseria dumasiana sp. nov.），模式菌株为93087（=DSM 104677=LMG 30012）。
