鉴定植物 K 通道 KAT1 和 AKT2 在酿酒酵母和非洲爪蟾卵母细胞中功能的相关区域。
Identification of regions responsible for the function of the plant K channels KAT1 and AKT2 in Saccharomyces cerevisiae and Xenopus laevis oocytes.
机构信息
a Department of Biomolecular Engineering , Graduate School of Engineering, Tohoku University , Sendai , Japan.
b Bioscience and Biotechnology Center , Nagoya University , Nagoya , Japan.
出版信息
Channels (Austin). 2017 Nov 2;11(6):510-516. doi: 10.1080/19336950.2017.1372066. Epub 2017 Oct 5.
The Arabidopsis K channel KAT1 complements in K-limited medium the growth of the K uptake defective Saccharomyces cerevisiae mutant strain CY162, while another K channel, AKT2, does not. To gain insight into the structural basis for this difference, we constructed 12 recombinant chimeric channels from these two genes. When expressed in CY162, only three of these chimeras fully rescued the growth of CY162 under K-limited conditions. We conclude that the transmembrane core region of KAT1 is important for its activity in S. cerevisiae. This involves not only the pore region but also parts of its voltage-sensor domain.
拟南芥 K 通道 KAT1 在低钾培养基中补充了钾吸收缺陷型酿酒酵母突变株 CY162 的生长，而另一种 K 通道 AKT2 则没有。为了深入了解这种差异的结构基础，我们从这两个基因构建了 12 种重组嵌合通道。当在 CY162 中表达时，这些嵌合体中只有三种完全挽救了 CY162 在低钾条件下的生长。我们得出结论，KAT1 的跨膜核心区域对其在酿酒酵母中的活性很重要。这不仅涉及孔区域，还涉及其电压传感器结构域的部分区域。
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