a The University of Texas at Austin, College of Pharmacy , Division of Molecular Pharmaceutics and Drug Delivery , Austin , TX , USA.
b Texas Tech University Health Sciences Center El Paso , Department of Biomedical Sciences, Center of Emphasis in Infectious Diseases , El Paso , TX , USA.
Hum Vaccin Immunother. 2017 Nov 2;13(11):2688-2694. doi: 10.1080/21645515.2017.1365995. Epub 2017 Sep 21.
Some insoluble aluminum salts are commonly used in injectable vaccines as adjuvants to accelerate, prolong, or enhance the antigen-specific immune responses. Data from previous studies testing the nasal mucosal vaccine adjuvant activity of aluminum salts are conflicting. The present study is designed to further assess the feasibility of using aluminum salts in injectable vaccines as nasal mucosal vaccine adjuvants. Using Alhydrogel®, the international scientific standard of aluminum (oxy)hydroxide gels, and ovalbumin or 3 × M2e-HA2, a synthetic influenza virus fusion protein, as antigens, we showed in a mouse model that when dosed intranasally Alhydrogel® enables antigens adsorbed on it to induce stronger antigen-specific immune responses in both serum samples (e.g., specific IgG) and nasal and lung mucosal secretions (i.e., specific IgA) in all immunized mice, as compared with nasal immunization with the antigens alone. Rerouting insoluble aluminum salts in injectable vaccines may represent a viable approach for (nasal) mucosal vaccine adjuvant discovery.
一些不溶性铝盐通常被用作可注射疫苗中的佐剂，以加速、延长或增强抗原特异性免疫反应。先前测试铝盐作为鼻黏膜疫苗佐剂活性的研究数据存在矛盾。本研究旨在进一步评估将铝盐用作可注射疫苗中的鼻黏膜疫苗佐剂的可行性。本研究使用铝（氧）氢氧化物凝胶的国际科学标准 Alhydrogel®和卵清蛋白或 3×M2e-HA2（一种合成流感病毒融合蛋白）作为抗原，在小鼠模型中表明，当经鼻内给予 Alhydrogel®时，与单独用抗原经鼻免疫相比，吸附在其上的抗原能够诱导所有免疫小鼠的血清样本（例如，特异性 IgG）和鼻及肺黏膜分泌物（即，特异性 IgA）中更强的抗原特异性免疫反应。将可注射疫苗中的不溶性铝盐重定向可能代表一种可行的（鼻）黏膜疫苗佐剂发现方法。
