通过基质辅助激光解吸电离飞行时间质谱直接从硫乙醇酸盐肉汤中鉴定微生物。
Direct identification of microorganisms from thioglycolate broth by MALDI-TOF MS.
作者信息
Arca-Suárez Jorge, Galán-Sánchez Fátima, Marin-Casanova Pilar, Rodríguez-Iglesias Manuel A
机构信息
Laboratorio de Microbiología, Hospital Universitario Puerta del Mar, Cádiz, Spain.
Área de Microbiología, Universidad de Cádiz, Cádiz, Spain.
出版信息
PLoS One. 2017 Sep 21;12(9):e0185229. doi: 10.1371/journal.pone.0185229. eCollection 2017.
Abstract
We developed an easy MALDI-TOF MS-based assay to identify microorganisms directly from thioglycolate broth. A total of 101 positive thioglycolate broths inoculated with 15 different kinds of samples were evaluated. In 91 samples (90.1%), direct MALDI-TOF MS identifications were the same as those obtained after conventional laboratory procedures including subcultures. In 10 samples misidentified by direct processing, yeasts or mixed cultures grew in the thioglycolate subcultures, or high cellular debris hampered a correct analysis. This rapid method can provide a fast, clinically- relevant species-level identification without disturbing the daily workflow in clinical microbiology laboratories.
摘要
我们开发了一种基于基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）的简易检测方法，可直接从硫乙醇酸盐肉汤中鉴定微生物。共评估了接种15种不同样本的101份硫乙醇酸盐肉汤阳性样本。在91份样本（90.1%）中，直接MALDI-TOF MS鉴定结果与包括传代培养在内的传统实验室程序获得的结果相同。在10份直接处理时鉴定错误的样本中，硫乙醇酸盐传代培养物中生长出酵母或混合培养物，或者高细胞碎片妨碍了正确分析。这种快速方法可以在不干扰临床微生物学实验室日常工作流程的情况下，提供快速、临床相关的菌种水平鉴定。
相似文献
1
Direct identification of microorganisms from thioglycolate broth by MALDI-TOF MS.
PLoS One. 2017 Sep 21;12(9):e0185229. doi: 10.1371/journal.pone.0185229. eCollection 2017.
2
MALDI-TOF mass spectrometry following short incubation on a solid medium is a valuable tool for rapid pathogen identification from positive blood cultures.
Int J Med Microbiol. 2015 Jun-Aug;305(4-5):469-79. doi: 10.1016/j.ijmm.2015.04.004. Epub 2015 Apr 27.
3
Microorganisms direct identification from blood culture by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
Clin Microbiol Infect. 2011 Apr;17(4):546-51. doi: 10.1111/j.1469-0691.2010.03257.x.
4
Real-time identification of bacteria and Candida species in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
J Clin Microbiol. 2010 May;48(5):1542-8. doi: 10.1128/JCM.02485-09. Epub 2010 Mar 17.
5
Identification of microorganisms grown on chromogenic media by MALDI-TOF MS.
J Microbiol Methods. 2017 May;136:17-20. doi: 10.1016/j.mimet.2017.03.001. Epub 2017 Mar 4.
6
Improvement of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry identification of difficult-to-identify bacteria and its impact in the workflow of a clinical microbiology laboratory.
Diagn Microbiol Infect Dis. 2014 May;79(1):1-6. doi: 10.1016/j.diagmicrobio.2014.01.021. Epub 2014 Jan 30.
7
Direct identification of clinical pathogens from liquid culture media by MALDI-TOF MS analysis.
Clin Microbiol Infect. 2018 Jun;24(6):624-629. doi: 10.1016/j.cmi.2017.09.010. Epub 2017 Sep 27.
8
Rapid identification of microorganisms from positive blood cultures by MALDI-TOF mass spectrometry subsequent to very short-term incubation on solid medium.
Clin Microbiol Infect. 2014 Oct;20(10):1001-6. doi: 10.1111/1469-0691.12640. Epub 2014 May 15.
9
Matrix-assisted laser desorption ionization-time of flight mass spectrometry for the identification of bacteria and yeasts in a clinical microbiological laboratory: a review.
Acta Clin Belg. 2011 Jul-Aug;66(4):267-73. doi: 10.2143/ACB.66.4.2062567.
10
Rapid Identification of Microorganisms from Positive Blood Culture by MALDI-TOF MS After Short-Term Incubation on Solid Medium.
Curr Microbiol. 2017 Jan;74(1):97-102. doi: 10.1007/s00284-016-1161-2. Epub 2016 Nov 17.
引用本文的文献
1
Spectral Precision: Recent Advances in Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for Pathogen Detection and Resistance Profiling.
Microorganisms. 2025 Jun 25;13(7):1473. doi: 10.3390/microorganisms13071473.
2
Large-Scale Samples Based Rapid Detection of Ciprofloxacin Resistance in Using Machine Learning Methods.
Front Microbiol. 2022 Mar 8;13:827451. doi: 10.3389/fmicb.2022.827451. eCollection 2022.
3
Current status of MALDI-TOF mass spectrometry in clinical microbiology.
J Food Drug Anal. 2019 Apr;27(2):404-414. doi: 10.1016/j.jfda.2019.01.001. Epub 2019 Jan 31.
4
Susceptibility Testing of Bacteria Using Maldi-Tof Mass Spectrometry.
Front Microbiol. 2018 Aug 6;9:1744. doi: 10.3389/fmicb.2018.01744. eCollection 2018.
5
Validation of a novel automatic deposition of bacteria and yeasts on MALDI target for MALDI-TOF MS-based identification using MALDI Colonyst robot.
PLoS One. 2017 Dec 29;12(12):e0190038. doi: 10.1371/journal.pone.0190038. eCollection 2017.
本文引用的文献
1
Executive Summary: Implementing an Antibiotic Stewardship Program: Guidelines by the Infectious Diseases Society of America and the Society for Healthcare Epidemiology of America.
Clin Infect Dis. 2016 May 15;62(10):1197-1202. doi: 10.1093/cid/ciw217.
2
MALDI-TOF MS performance compared to direct examination, culture, and 16S rDNA PCR for the rapid diagnosis of bone and joint infections.
Eur J Clin Microbiol Infect Dis. 2016 May;35(5):857-66. doi: 10.1007/s10096-016-2608-x. Epub 2016 Mar 4.
3
Propionibacterium acnes: from commensal to opportunistic biofilm-associated implant pathogen.
Clin Microbiol Rev. 2014 Jul;27(3):419-40. doi: 10.1128/CMR.00092-13.
4
Evaluation of three rapid diagnostic methods for direct identification of microorganisms in positive blood cultures.
J Clin Microbiol. 2014 Jul;52(7):2521-9. doi: 10.1128/JCM.00529-14. Epub 2014 May 7.
5
Direct application of MALDI-TOF mass spectrometry to cerebrospinal fluid for rapid pathogen identification in a patient with bacterial meningitis.
Clin Chim Acta. 2014 Aug 5;435:59-61. doi: 10.1016/j.cca.2014.04.024. Epub 2014 May 4.
6
Matrix-assisted laser desorption ionization-time of flight mass spectrometry: a fundamental shift in the routine practice of clinical microbiology.
Clin Microbiol Rev. 2013 Jul;26(3):547-603. doi: 10.1128/CMR.00072-12.
7
Identification of Gram-positive cocci by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry: comparison of different preparation methods and implementation of a practical algorithm for routine diagnostics.
J Clin Microbiol. 2013 Jun;51(6):1834-40. doi: 10.1128/JCM.02654-12. Epub 2013 Apr 3.
8
Direct bacterial identification in positive blood cultures by use of two commercial matrix-assisted laser desorption ionization-time of flight mass spectrometry systems.
J Clin Microbiol. 2013 Jun;51(6):1733-9. doi: 10.1128/JCM.03259-12. Epub 2013 Mar 20.
9
Advances in identification of clinical yeast isolates by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry.
J Clin Microbiol. 2013 May;51(5):1359-66. doi: 10.1128/JCM.03105-12. Epub 2013 Feb 20.
10
Identification of blood culture isolates directly from positive blood cultures by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry and a commercial extraction system: analysis of performance, cost, and turnaround time.
J Clin Microbiol. 2012 Oct;50(10):3324-8. doi: 10.1128/JCM.01479-12. Epub 2012 Aug 8.
Zotero 插件