通过基质辅助激光解吸电离飞行时间质谱直接从硫乙醇酸盐肉汤中鉴定微生物。
Direct identification of microorganisms from thioglycolate broth by MALDI-TOF MS.
作者信息
Arca-Suárez Jorge, Galán-Sánchez Fátima, Marin-Casanova Pilar, Rodríguez-Iglesias Manuel A
机构信息
Laboratorio de Microbiología, Hospital Universitario Puerta del Mar, Cádiz, Spain.
Área de Microbiología, Universidad de Cádiz, Cádiz, Spain.
出版信息
PLoS One. 2017 Sep 21;12(9):e0185229. doi: 10.1371/journal.pone.0185229. eCollection 2017.
Abstract
We developed an easy MALDI-TOF MS-based assay to identify microorganisms directly from thioglycolate broth. A total of 101 positive thioglycolate broths inoculated with 15 different kinds of samples were evaluated. In 91 samples (90.1%), direct MALDI-TOF MS identifications were the same as those obtained after conventional laboratory procedures including subcultures. In 10 samples misidentified by direct processing, yeasts or mixed cultures grew in the thioglycolate subcultures, or high cellular debris hampered a correct analysis. This rapid method can provide a fast, clinically- relevant species-level identification without disturbing the daily workflow in clinical microbiology laboratories.
摘要
我们开发了一种基于基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）的简易检测方法，可直接从硫乙醇酸盐肉汤中鉴定微生物。共评估了接种15种不同样本的101份硫乙醇酸盐肉汤阳性样本。在91份样本（90.1%）中，直接MALDI-TOF MS鉴定结果与包括传代培养在内的传统实验室程序获得的结果相同。在10份直接处理时鉴定错误的样本中，硫乙醇酸盐传代培养物中生长出酵母或混合培养物，或者高细胞碎片妨碍了正确分析。这种快速方法可以在不干扰临床微生物学实验室日常工作流程的情况下，提供快速、临床相关的菌种水平鉴定。
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