Rodionova Natalia N, Allakhverdiev Elvin S, Maksimov Georgy V
Faculty of Biology, M.V. Lomonosov Moscow State University, Moscow, Russia.
Faculty of Fundamental Medicine, M.V. Lomonosov Moscow State University, Moscow, Russia.
PLoS One. 2017 Sep 21;12(9):e0185170. doi: 10.1371/journal.pone.0185170. eCollection 2017.
Raman, NMR and EPR spectroscopy and electrophysiology methods were used to investigate the excitability and the packaging of myelin lipid layers and its viscosity during nerve exposure to pronase E. It was established that during exposure of nerve to pronase E the action potential (AP) conduction velocity and the Schwann cell (SC) (or myelin) water ordering increases, but the nerve myelin refractive index and internode incisions numbers decrease. This effect included two periods-short- and long-time period, probably, because the first one depends on SC protein changes and the second one-on the nerve fiber internode demyelination. It was concluded that high electrical resistance of myelin, which is important for a series of AP conduction velocity, not only depends on nerve fiber diameter and the myelin lipid composition, but also on the regularity of myelin lipid fatty acids and myelin lipid layer packing during the axoglial interaction.
利用拉曼光谱、核磁共振光谱、电子顺磁共振光谱以及电生理方法，研究了在神经暴露于链霉蛋白酶E的过程中，髓磷脂脂质层的兴奋性、包装情况及其黏度。研究发现，在神经暴露于链霉蛋白酶E的过程中，动作电位（AP）传导速度和施万细胞（SC）（或髓磷脂）的水有序性增加，但神经髓磷脂折射率和结间切口数量减少。这种效应包括两个阶段——短期和长期，这可能是因为第一个阶段取决于施万细胞蛋白质的变化，而第二个阶段取决于神经纤维结间脱髓鞘。研究得出结论，髓磷脂的高电阻对一系列动作电位传导速度很重要，它不仅取决于神经纤维直径和髓磷脂脂质组成，还取决于轴突-神经胶质相互作用过程中髓磷脂脂质脂肪酸的规则性和髓磷脂脂质层的堆积情况。
