Skak-Nielsen T, Holst J J, Baldissera F G, Poulsen S S
Institute of Medical Physiology C, Panum Institute, University of Copenhagen, Denmark.
Regul Pept. 1987 Nov;19(3-4):183-95. doi: 10.1016/0167-0115(87)90275-8.
Antisera against 5 different regions of the entire prosomatostatin molecule were used for immunohistochemical mapping of prosomatostatin-containing structures in the pig gastrointestinal tract, and for radioimmunological and chromatographical analysis of the products of prosomatostatin in extracts of ileal mucosa. The latter showed that the antisera were capable of identifying components containing N-terminal as well as C-terminal parts of prosomatostatin. Endocrine cells were identified with all antisera in most parts of the gastrointestinal tract, and varicose nerve fibres were observed in all parts of the small intestine but not in the stomach and the colon. The colon contained very few immunoreactive structures. Immunoreactive nerve cell bodies were found in the submucous plexus of the small intestine. All immunoreactive endocrine cells in the stomach and the duodenum and all immunoreactive nerves were stained by all 5 antisera whereas the small intestinal endocrine cells did not stain for the most N-terminal region of prosomatostatin. The results suggest that all gastrointestinal somatostatin is derived from the same precursor molecule, which, however, in the small intestinal endocrine cells is processed differently from that of the other tissues.
使用针对整个前生长抑素分子5个不同区域的抗血清，对猪胃肠道中含前生长抑素的结构进行免疫组织化学定位，并对回肠黏膜提取物中的前生长抑素产物进行放射免疫和色谱分析。后者表明，这些抗血清能够识别含有前生长抑素N端和C端部分的成分。在胃肠道的大部分区域，所有抗血清都能识别内分泌细胞，在小肠的所有部位都观察到了曲张神经纤维，但在胃和结肠中未观察到。结肠中含有的免疫反应性结构极少。在小肠的黏膜下神经丛中发现了免疫反应性神经细胞体。胃和十二指肠中的所有免疫反应性内分泌细胞以及所有免疫反应性神经都被所有5种抗血清染色，而小肠内分泌细胞对前生长抑素最N端区域未染色。结果表明，所有胃肠道生长抑素均来自同一前体分子，然而，在小肠内分泌细胞中，其加工方式与其他组织不同。
