Holst J J, Baldissera F G, Bersani M, Skak-Nielsen T, Seier-Poulsen S, Nielsen O V
Institute of Medical Physiology C, Panum Institute, University of Copenhagen, Denmark.
Pancreas. 1988;3(6):653-61. doi: 10.1097/00006676-198812000-00005.
Antisera and radioimmunoassays against five different regions of prosomatostatin (proSS) were used for chromatographical analysis and for immunohistochemical mapping of the products of proSS in the pig pancreas. Secreted products of proSS were studied by analysis of effluent from isolated perfused pig pancreas obtained during isoproterenol stimulation. All cells that were stained with one antiserum also stained with the other antisera. Immunoreactive nerves were not observed. Isoproterenol increased equally the secretion of proSS 20-36, proSS 65-76, and proSS 79-92 immunoreactivity. The major molecular forms identified in pancreatic extracts and released from the pancreas were proSS 79-92; proSS 65-76; an N-terminally extended form of proSS 65-76; and two larger forms comprising the proSS 20-36 sequence (but not the 1-13 sequence) with and without the proSS 65-76 sequence. ProSS 1-10, 1-32 and 65-92 (somatostatin 28) were not identified.
针对前生长抑素（proSS）五个不同区域的抗血清和放射免疫分析被用于猪胰腺中proSS产物的色谱分析和免疫组织化学定位。通过分析异丙肾上腺素刺激期间从分离的灌注猪胰腺流出的物质来研究proSS的分泌产物。用一种抗血清染色的所有细胞也能用其他抗血清染色。未观察到免疫反应性神经。异丙肾上腺素同等程度地增加了proSS 20 - 36、proSS 65 - 76和proSS 79 - 92免疫反应性的分泌。在胰腺提取物中鉴定出并从胰腺释放的主要分子形式为proSS 79 - 92；proSS 65 - 76；proSS 65 - 76的N端延伸形式；以及两种较大的形式，包含有和没有proSS 65 - 76序列的proSS 20 - 36序列（但不包含1 - 13序列）。未鉴定出proSS 1 - 10、1 - 32和65 - 92（生长抑素28）。
