Pariat C, Courtois P, Cambar J, Piriou A, Bouquet S
Institut d'Etudes des Médicaments, U.E.R. de Médecine et de Pharmacie, Poitiers, France.
Toxicol Lett. 1988 Feb;40(2):175-82. doi: 10.1016/0378-4274(88)90159-2.
The hypothesis that sublethal doses of aminoglycosides cause renal tubule disorders resulting in changes of urine enzyme levels was investigated. The renal status following injection of a single sublethal dose of gentamicin (200 mg/kg) at different times during a 24 h cycle was studied. Increased excretion of gamma-glutamyl transferase, alanine aminopeptidase and N-acetyl-beta-D-glucosaminidase, used clinically as markers for tubule toxicity of aminoglycosides, was maximal when gentamicin was administered to rats at 2 p.m. and was minimal when injected at 8 p.m. These significant differences in enzyme excretion as a function of injection time are correlated with the concentration of gentamicin in the urine and in the renal cortex.
