Panhwar Abdul Haleem, Tuzen Mustafa, Kazi Tasneem Gul
University of Sindh, National Centre of Excellence in Analytical Chemistry, Jamshoro 76080, Pakistan.
Gaziosmanpasa University, Faculty of Science and Arts, Chemistry Department, 60250 Tokat, Turkey.
J AOAC Int. 2018 Jul 1;101(4):1183-1189. doi: 10.5740/jaoacint.17-0286. Epub 2017 Sep 21.
An innovative and effective digestion method based on choline chloride (ChCl)-oxalic acid (Ox) deep eutectic solvent (DES) was proposed for the determination of Se and As in fish samples via electrothermal atomic absorption spectrometry (ETAAS). The impacts of different variables, including the composition and volume of ChCl-Ox, temperature, and acid addition, on analyte recovery were studied for optimization. In this procedure, an 80 mg sample was dissolved in a 1:2 molar ratio of ChCl-Ox at 105°C for 40 min, with the subsequent addition of 4.0 mL HNO3 (1.0 M) and further heating at the same temperature for about 5 min. Next, centrifugation was applied, and the supernatant solution was filtered, diluted to a known volume, and measured by ETAAS. The accuracy of the developed method was tested using a Standard Reference Material (NIST SRM 1946 Lake Superior Fish Tissue). The proposed DES-based digestion method was successfully applied to the simultaneous extraction of Se and As from fish samples.
提出了一种基于氯化胆碱（ChCl）-草酸（Ox）低共熔溶剂（DES）的创新且有效的消解方法，用于通过电热原子吸收光谱法（ETAAS）测定鱼样中的硒和砷。研究了不同变量，包括ChCl-Ox的组成和体积、温度以及酸的添加量对分析物回收率的影响，以进行优化。在此过程中，将80 mg样品溶解于1:2摩尔比的ChCl-Ox中，在105°C下加热40分钟，随后加入4.0 mL硝酸（1.0 M），并在相同温度下进一步加热约5分钟。接着进行离心，将上清液过滤、稀释至已知体积，然后用ETAAS进行测量。使用标准参考物质（NIST SRM 1946苏必利尔湖鱼类组织）测试了所开发方法的准确性。所提出的基于DES的消解方法成功应用于从鱼样中同时提取硒和砷。
