原核生物特异性尿苷二磷酸糖的化学酶法合成及应用
Chemoenzymatic Synthesis and Applications of Prokaryote-Specific UDP-Sugars.
作者信息
Zamora Cristina Y, Schocker Nathaniel S, Chang Michelle M, Imperiali Barbara
机构信息
Massachusetts Institute of Technology, Cambridge, MA, United States.
Massachusetts Institute of Technology, Cambridge, MA, United States.
出版信息
Methods Enzymol. 2017;597:145-186. doi: 10.1016/bs.mie.2017.06.003. Epub 2017 Jul 5.
Abstract
This method describes the chemoenzymatic synthesis of several nucleotide sugars, which are essential substrates in the biosynthesis of prokaryotic N- and O-linked glycoproteins. Protein glycosylation is now known to be widespread in prokaryotes and proceeds via sequential action of several enzymes, utilizing both common and modified prokaryote-specific sugar nucleotides. The latter, which include UDP-hexoses such as UDP-diNAc-bacillosamine (UDP-diNAcBac), UDP-diNAcAlt, and UDP-2,3-diNAcManA, are also important components of other bacterial and archaeal glycoconjugates. The ready availability of these "high-value" intermediates will enable courses of study into inhibitor screening, glycoconjugate biosynthesis pathway discovery, and unnatural carbohydrate incorporation toward metabolic engineering.
摘要
该方法描述了几种核苷酸糖的化学酶促合成，这些核苷酸糖是原核生物N-连接和O-连接糖蛋白生物合成中的必需底物。现在已知蛋白质糖基化在原核生物中广泛存在，并通过几种酶的顺序作用进行，利用常见的和修饰的原核生物特异性糖核苷酸。后者包括UDP-己糖，如UDP-二乙酰基杆菌胺（UDP-diNAcBac）、UDP-二乙酰基-alt和UDP-2,3-二乙酰基甘露糖胺A，也是其他细菌和古细菌糖缀合物的重要组成部分。这些“高价值”中间体的现成可得性将有助于开展抑制剂筛选、糖缀合物生物合成途径发现以及非天然碳水化合物掺入代谢工程等研究课程。
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