Rejzek Martin, Hill Lionel, Hems Edward S, Kuhaudomlarp Sakonwan, Wagstaff Ben A, Field Robert A
John Innes Centre, Norwich, United Kingdom.
John Innes Centre, Norwich, United Kingdom.
Methods Enzymol. 2017;597:209-238. doi: 10.1016/bs.mie.2017.06.005. Epub 2017 Jul 23.
Sugar nucleotides are essential building blocks for the glycobiology of all living organisms. Detailed information on the types of sugar nucleotides present in a particular cell and how they change as a function of metabolic, developmental, or disease status is vital. The extraction, identification, and quantification of sugar nucleotides in a given sample present formidable challenges. In this chapter, currently used techniques for sugar nucleotide extraction from cells, separation from complex biological matrices, and detection by optical and mass spectrometry methods are discussed.
糖核苷酸是所有生物体糖生物学的基本组成部分。关于特定细胞中存在的糖核苷酸类型以及它们如何随代谢、发育或疾病状态而变化的详细信息至关重要。在给定样品中提取、鉴定和定量糖核苷酸面临巨大挑战。在本章中，将讨论目前用于从细胞中提取糖核苷酸、从复杂生物基质中分离糖核苷酸以及通过光学和质谱方法进行检测的技术。
