Ezeasor D N, Singh A
Department of Veterinary Anatomy, University of Nigeria, Nsukka.
Am J Vet Res. 1987 Dec;48(12):1736-45.
Light and electron microscopy revealed an age-related progression of alterations of Sertoli cells in the intra-abdominal and scrotal testes of unilaterally cryptorchid West African dwarf goats between the ages of 1 and 30 months. Alterations in the scrotal testis were, however, maturational and included differentiation of Sertoli-to-Sertoli cell junctional specializations, profusion of smooth endoplasmic reticulum, convolution of nuclear profiles, development of vacuolar components of the nucleolus, and an overall change in cell shape in response to proliferation of germinal cells. Corresponding features were observed in Sertoli cells of the contralateral intra-abdominal testis, but the cytoplasmic features were transient because the cells degenerated progressively. Early changes included segregation of the smooth endoplasmic reticulum into compact masses composed of dense, narrow cisternae, dilatation of the rough endoplasmic reticulum cisternae into large, irregular profiles, atrophy of the Golgi complex, and accumulation of lipid droplets and lipofuscin granules. Many of these organelles and inclusions no longer were obvious in Sertoli cells of 12- to 15-month-old goats; rather, intracellular vacuoles and dilated intercellular spaces had become common. In the 24- to 30-month-old goats, Sertoli cells in the intra-abdominal testis contained mostly microfilaments and basally located mitochondria with circular cristae in dense matrices. The Sertoli-to-Sertoli cell junctional specializations were structurally intact. These results indicated that, in spite of the unfavorable intra-abdominal environment, Sertoli cells of the intra-abdominal testis, before their degeneration, had developed features similar to those of the scrotal testis.
光镜和电镜检查显示，1至30月龄的单侧隐睾西非矮山羊腹内和阴囊内睾丸的支持细胞存在与年龄相关的变化进程。然而，阴囊内睾丸的变化属于成熟性变化，包括支持细胞间连接特化的分化、滑面内质网丰富、核轮廓卷曲、核仁液泡成分的发育以及因生精细胞增殖导致的细胞形状整体改变。在对侧腹内睾丸的支持细胞中观察到了相应特征，但细胞质特征是短暂的，因为细胞逐渐退化。早期变化包括滑面内质网分离成由致密、狭窄的池组成的紧密团块、粗面内质网池扩张成大的不规则轮廓、高尔基体萎缩以及脂滴和脂褐素颗粒的积累。在12至15月龄山羊的支持细胞中，许多这些细胞器和内含物不再明显；相反，细胞内空泡和扩张的细胞间隙变得常见。在24至30月龄的山羊中，腹内睾丸的支持细胞主要含有微丝和基部的线粒体，线粒体嵴呈圆形，基质致密。支持细胞间连接特化结构完整。这些结果表明，尽管腹内环境不利，但腹内睾丸的支持细胞在退化之前已发展出与阴囊内睾丸相似的特征。
