Key Laboratory of Pollution Processes and Environmental Criteria of Ministry of Education of the People's Republic of China, College of Environmental Science and Engineering, Nankai University, Tianjin, 300071, People's Republic of China.
Centre for Urban Transport Emission Research, College of Environmental Science and Engineering, Nankai University, Tianjin, 300071, People's Republic of China.
Sci Rep. 2017 Sep 21;7(1):12114. doi: 10.1038/s41598-017-12503-3.
Two kinds of biochars, one derived from corn straw and one from pig manure, were studied as carriers of a mutant genotype from Bacillus subtilis (B38) for heavy metal contaminated soil remediation. After amendment with biochar, the heavy metal bioavailability decreased. Moreover, the heavy metal immobilization ability of the biochar was enhanced by combining it with B38. The simultaneous application of B38 and pig manure-derived biochar exhibited a superior effect on the promotion of plant growth and the immobilization of heavy metals in soil. The plant biomass increased by 37.9% and heavy metal concentrations in the edible part of lettuce decreased by 69.9-96.1%. The polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) profiles revealed that pig manure-derived biochar could enhance the proliferation of both exotic B38 and native microbes. These results suggest that B38 carried by pig manure-derived biochar may be a promising candidate for the remediation of soils contaminated by multiple heavy metals.
两种生物炭，一种来源于玉米秸秆，另一种来源于猪粪，被研究为用于修复重金属污染土壤的枯草芽孢杆菌（B38）突变基因型的载体。用生物炭进行改良后，重金属的生物利用度降低。此外，通过将 B38 与生物炭结合，生物炭的重金属固定能力得到增强。同时应用 B38 和猪粪衍生生物炭对促进植物生长和固定土壤中的重金属具有优越的效果。植物生物量增加了 37.9%，生菜可食用部分的重金属浓度降低了 69.9-96.1%。聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）图谱显示，猪粪衍生生物炭可以增强外来 B38 和本地微生物的增殖。这些结果表明，猪粪衍生生物炭携带的 B38 可能是修复多种重金属污染土壤的有前途的候选物。
