Gaziev Azhub, Abdullaev Serazhutdin, Minkabirova Gulchachak, Kamenskikh Kristina
Institute of Theoretical and Experimental Biophysics, RAS, Pushino, Moscow Region, Russia.
J Circ Biomark. 2016 Oct 25;5:1849454416670782. doi: 10.1177/1849454416670782. eCollection 2016 Jan-Dec.
Activation of cell death in mammals can be assessed by an increase of an amount of cell-free DNA (cf-DNA) in urine or plasma. We investigated the excretion of cf nuclear DNA (nDNA) and cf mitochondrial DNA (mtDNA) in the urine of rats 3 and 24 months in age after X-irradiation and metformin administration. Analyses showed that prior to treatment, the amount of cf-nDNA was 40% higher and cf-mtDNA was 50% higher in the urine of aged rats compared to that of young animals. At 12 h after irradiation, the content of cf-nDNA and cf-mtDNA in the urine of young rats was increased by 200% and 460%, respectively, relative to the control, whereas in the urine of aged rats, it was 250% and 720% higher. After 6 h following metformin administration, the amount of cf-nDNA and cf-mtDNA in the urine of young rats was elevated by 25% and 55% and by 50% and 160% in the urine of aged rats. Thus, these preliminary data suggest that X-rays and metformin cause a significant increase of cf-DNA in the urine of older rats caused by the active cell death in tissues. These results also suggest that metformin possibly initiates the death of the cells containing structural and functional abnormalities.
哺乳动物细胞死亡的激活可以通过尿液或血浆中游离细胞DNA（cf-DNA）量的增加来评估。我们研究了3月龄和24月龄大鼠在接受X射线照射和给予二甲双胍后尿液中cf核DNA（nDNA）和cf线粒体DNA（mtDNA）的排泄情况。分析表明，在治疗前，老年大鼠尿液中的cf-nDNA量比年轻动物高40%，cf-mtDNA量高50%。照射后12小时，年轻大鼠尿液中cf-nDNA和cf-mtDNA的含量相对于对照组分别增加了200%和460%，而老年大鼠尿液中的含量则分别高出250%和720%。给予二甲双胍6小时后，年轻大鼠尿液中cf-nDNA和cf-mtDNA的量分别升高了25%和55%，老年大鼠尿液中分别升高了50%和160%。因此，这些初步数据表明，X射线和二甲双胍会导致老年大鼠尿液中cf-DNA显著增加，这是由组织中的活跃细胞死亡引起的。这些结果还表明，二甲双胍可能引发含有结构和功能异常的细胞死亡。
