小鼠 Müller 细胞的分离与培养。
Mouse Müller Cell Isolation and Culture.
作者信息
Liu Xiao, Tang Luosheng, Liu Yongqing
机构信息
Department of Ophthalmology and Visual Sciences, University of Louisville, Louisville, USA.
Department of Ophthalmology, Second Xiangya Hospital of Central South University, Changsha, China.
出版信息
Bio Protoc. 2017 Aug 5;7(15). doi: 10.21769/BioProtoc.2429.
Abstract
Müller cells are the major supportive and protective glial cells across the retina. Unlike in fish, they have lost the capacity to regenerate the retina in mammals. But, mammalian Müller cells still retain certain retinal stem cell properties with various degree of self-renewal and differentiation potentials, and thereby held a merit in cell-based therapies for treating retinal degeneration diseases. In our laboratory, we use an enzymatic procedure to isolate, purify, and culture mouse Müller cells.
摘要
米勒细胞是视网膜中主要的支持性和保护性神经胶质细胞。与鱼类不同，在哺乳动物中它们已经失去了视网膜再生能力。但是，哺乳动物的米勒细胞仍然保留了一定的视网膜干细胞特性，具有不同程度的自我更新和分化潜能，因此在基于细胞的视网膜退行性疾病治疗中具有优势。在我们实验室，我们采用酶法来分离、纯化和培养小鼠米勒细胞。
相似文献
1
Mouse Müller Cell Isolation and Culture.小鼠 Müller 细胞的分离与培养。
Bio Protoc. 2017 Aug 5;7(15). doi: 10.21769/BioProtoc.2429.
2
Müller glia: Stem cells for generation and regeneration of retinal neurons in teleost fish.缪勒胶质细胞：硬骨鱼视网膜神经元生成与再生的干细胞。
Prog Retin Eye Res. 2014 May;40:94-123. doi: 10.1016/j.preteyeres.2013.12.007. Epub 2014 Jan 8.
3
Insights on the Regeneration Potential of Müller Glia in the Mammalian Retina.对哺乳动物视网膜中 Müller 胶质细胞再生潜力的见解。
Cells. 2021 Jul 31;10(8):1957. doi: 10.3390/cells10081957.
4
Production of neurospheres from mammalian Müller cells in culture.在培养中从哺乳动物米勒细胞生成神经球。
Neurosci Lett. 2007 Jun 21;421(1):22-6. doi: 10.1016/j.neulet.2007.04.073. Epub 2007 May 16.
5
Molecular characterization of retinal stem cells and their niches in adult zebrafish.成年斑马鱼视网膜干细胞及其生态位的分子特征
BMC Dev Biol. 2006 Jul 26;6:36. doi: 10.1186/1471-213X-6-36.
6
Müller glial cells induce stem cell properties in retinal progenitors in vitro and promote their further differentiation into photoreceptors.Müller 胶质细胞在体外诱导视网膜祖细胞产生干细胞特性，并促进其进一步分化为光感受器。
J Neurosci Res. 2012 Feb;90(2):407-21. doi: 10.1002/jnr.22747. Epub 2011 Oct 4.
7
Neural stem cell properties of Müller glia in the mammalian retina: regulation by Notch and Wnt signaling.哺乳动物视网膜中 Müller 胶质细胞的神经干细胞特性：Notch 和 Wnt 信号通路的调控
Dev Biol. 2006 Nov 1;299(1):283-302. doi: 10.1016/j.ydbio.2006.07.029. Epub 2006 Jul 29.
8
DNA Damage Response in Proliferating Müller Glia in the Mammalian Retina.哺乳动物视网膜中增殖性米勒胶质细胞的DNA损伤反应
Invest Ophthalmol Vis Sci. 2016 Mar;57(3):1169-82. doi: 10.1167/iovs.15-18101.
9
A Defective Crosstalk Between Neurons and Müller Glial Cells in the Retina Impairs the Regenerative Potential of Glial Stem Cells.视网膜中神经元与米勒胶质细胞之间的串扰缺陷会损害胶质干细胞的再生潜能。
Front Cell Neurosci. 2019 Jul 25;13:334. doi: 10.3389/fncel.2019.00334. eCollection 2019.
10
Focused ultrasound as a novel strategy for noninvasive gene delivery to retinal Müller glia.聚焦超声作为一种新型的非侵入性基因传递策略，用于视网膜 Müller 胶质细胞。
Theranostics. 2020 Feb 10;10(7):2982-2999. doi: 10.7150/thno.42611. eCollection 2020.
引用本文的文献
1
Characterization and neurogenic responses of primary and immortalized Müller glia.原代和永生化穆勒胶质细胞的特性及神经源性反应
Front Cell Dev Biol. 2025 May 9;13:1513163. doi: 10.3389/fcell.2025.1513163. eCollection 2025.
2
Age- and Sex-Specific Regulation of Serine Racemase in the Retina of an Alzheimer's Disease Mouse.阿尔茨海默病小鼠视网膜中丝氨酸消旋酶的年龄和性别特异性调节
Invest Ophthalmol Vis Sci. 2025 Jan 2;66(1):36. doi: 10.1167/iovs.66.1.36.
3
12-HETE activates Müller glial cells: The potential role of GPR31 and miR-29.12-羟基二十碳四烯酸激活 Müller 胶质细胞：GPR31 和 miR-29 的潜在作用
Prostaglandins Other Lipid Mediat. 2024 Apr;171:106805. doi: 10.1016/j.prostaglandins.2023.106805. Epub 2023 Dec 22.
4
Targeting the Mitochondrial Chaperone TRAP1 Alleviates Vascular Pathologies in Ischemic Retinopathy.靶向线粒体伴侣蛋白 TRAP1 可减轻缺血性视网膜病变中的血管病变。
Adv Sci (Weinh). 2024 Jan;11(2):e2302776. doi: 10.1002/advs.202302776. Epub 2023 Nov 20.
5
Transferrin Receptor-Targeted Iduronate-2-sulfatase Penetrates the Blood-Retinal Barrier and Improves Retinopathy in Mucopolysaccharidosis II Mice.转铁蛋白受体靶向艾杜糖-2-硫酸酯酶穿透血视网膜屏障并改善黏多糖贮积症 II 型小鼠的视网膜病变。
Mol Pharm. 2023 Nov 6;20(11):5901-5909. doi: 10.1021/acs.molpharmaceut.3c00736. Epub 2023 Oct 20.
6
IL-17A Enhances Retinal Neovascularization.IL-17A 增强视网膜新生血管形成。
Int J Mol Sci. 2023 Jan 16;24(2):1747. doi: 10.3390/ijms24021747.
7
Glial Bmal1 role in mammalian retina daily changes.胶质细胞 Bmal1 在哺乳动物视网膜昼夜节律变化中的作用。
Sci Rep. 2022 Dec 13;12(1):21561. doi: 10.1038/s41598-022-25783-1.
8
Model Systems for Studies Into Retinal Neuroprotection.视网膜神经保护研究的模型系统
Front Neurosci. 2022 Jul 7;16:938089. doi: 10.3389/fnins.2022.938089. eCollection 2022.
9
Characteristics of Whale Müller Glia in Primary and Immortalized Cultures.原代培养和永生化培养的鲸鱼米勒胶质细胞的特征。
Front Neurosci. 2022 Mar 14;16:854278. doi: 10.3389/fnins.2022.854278. eCollection 2022.
10
High glucose induces HSP47 expression and promotes the secretion of inflammatory factors through the IRE1α/XBP1/HIF-1α pathway in retinal Müller cells.高糖通过视网膜Müller细胞中的IRE1α/XBP1/HIF-1α途径诱导HSP47表达并促进炎症因子的分泌。
Exp Ther Med. 2021 Dec;22(6):1411. doi: 10.3892/etm.2021.10847. Epub 2021 Oct 7.
本文引用的文献
1
Sphere-Induced Rejuvenation of Swine and Human Müller Glia Is Primarily Caused by Telomere Elongation.
Stem Cells. 2017 Jun;35(6):1579-1591. doi: 10.1002/stem.2585. Epub 2017 Mar 5.
2
Müller glia as an active compartment modulating nervous activity in the vertebrate retina: neurotransmitters and trophic factors.缪勒胶质细胞作为调节脊椎动物视网膜神经活动的活跃部分：神经递质与营养因子。
Neurochem Res. 2008 Aug;33(8):1466-74. doi: 10.1007/s11064-008-9604-1. Epub 2008 Feb 14.
3
Müller cells in the healthy and diseased retina.健康和患病视网膜中的 Müller 细胞。
Prog Retin Eye Res. 2006 Jul;25(4):397-424. doi: 10.1016/j.preteyeres.2006.05.003. Epub 2006 Jul 12.
4
Müller glia are a potential source of neural regeneration in the postnatal chicken retina.缪勒胶质细胞是出生后鸡视网膜神经再生的潜在来源。
Nat Neurosci. 2001 Mar;4(3):247-52. doi: 10.1038/85090.
5
Light-induced rod and cone cell death and regeneration in the adult albino zebrafish (Danio rerio) retina.成年白化斑马鱼（Danio rerio）视网膜中光诱导的视杆和视锥细胞死亡与再生
J Neurobiol. 2000 Sep 5;44(3):289-307. doi: 10.1002/1097-4695(20000905)44:3<289::aid-neu1>3.0.co;2-h.
6
Biochemical studies of isolated glial (Müller) cells from the turtle retina.对来自乌龟视网膜的分离神经胶质（米勒）细胞的生化研究。
J Cell Biol. 1978 Sep;78(3):675-84. doi: 10.1083/jcb.78.3.675.
Zotero 插件