受体更新以及5-羟色胺对红头丽蝇、家蝇唾液腺和蛙皮肤上皮的作用。

Receptor turnover and the action of 5-hydroxytryptamine on the salivary glands of the blowfly Calliphora erythrocephala, the housefly Musca domestica and frog skin epithelium.

Dalton T

School of Biological Sciences, Queen Mary College, London, UK.

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1987;88(2):233-9. doi: 10.1016/0742-8413(87)90115-0.

Abstract

Continual stimulation of frog skin epithelium and the salivary glands of the insects Calliphora and Musca with 5-hydroxytryptamine (5-HT) leads to desensitisation, i.e. the tissue fails to respond to the application of further 5-HT. 2. Incubation of desensitised frog skin and Musca salivary glands with either N-acetyl neuraminic acid or inositol partially restored the 5-HT responses whilst incubation with a combination of N-acetyl neuraminic acid and inositol gave additive effects on the recovery of the 5-HT responses. 3. Incubation of desensitised salivary glands of Calliphora with inositol totally restored the 5-HT response whilst incubation with N-acetyl neuraminic acid had no effect. 4. It is concluded that desensitisation involves depletion of secondary messenger from the tissues coupled with receptor degradation and that considerable differences exist in the turnover of 5-HT receptors, the receptors in Musca salivary glands being highly labile, those in Calliphora salivary glands highly stable and those of frog skin epithelium being intermediate in their stability.

摘要

用5-羟色胺（5-HT）持续刺激青蛙皮肤上皮以及丽蝇和家蝇的唾液腺会导致脱敏，即组织对进一步施加的5-HT不再作出反应。2. 将脱敏的青蛙皮肤和家蝇唾液腺与N-乙酰神经氨酸或肌醇一起孵育，可部分恢复5-HT反应，而将其与N-乙酰神经氨酸和肌醇的组合一起孵育，对5-HT反应的恢复具有累加效应。3. 用肌醇孵育脱敏的丽蝇唾液腺可完全恢复5-HT反应，而用N-乙酰神经氨酸孵育则无效果。4. 得出的结论是，脱敏涉及组织中第二信使的耗竭以及受体降解，并且5-HT受体的更新存在相当大的差异，家蝇唾液腺中的受体高度不稳定，丽蝇唾液腺中的受体高度稳定，青蛙皮肤上皮中的受体稳定性处于中间水平。