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洛氏拟杆菌菌毛相关黏附素的定位与计数

Localization and enumeration of fimbria-associated adhesins of Bacteroides loescheii.

作者信息

Weiss E I, London J, Kolenbrander P E, Hand A R, Siraganian R

机构信息

Laboratory of Microbiology and Immunology, National Institute of Dental Research, Bethesda, Maryland 20892.

出版信息

J Bacteriol. 1988 Mar;170(3):1123-8. doi: 10.1128/jb.170.3.1123-1128.1988.

DOI:10.1128/jb.170.3.1123-1128.1988
PMID:2893786
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC210881/
Abstract

Monoclonal antibodies that specifically inhibit coaggregation between Bacteroides loescheii PK1295 and its two gram-positive partners Streptococcus sanguis 34 and Actinomyces israelii PK14 were used to enumerate and localize two distinct types of fimbria-associated adhesins on the surface of B. loescheii. Binding studies with radiolabeled monoclonal antibodies indicated that a maximum (Bmax calculated from Scatchard plots) of approximately 400 adhesin molecules specific for S. sanguis and 310 adhesin molecules specific for A. israelii reside on the surface of the cell. Immunoelectron microscopy revealed that the adhesins were not an integral part of the fimbrial subunit; rather, they were usually found on the distal portion of the structures arranged in a random fashion.

摘要

特异性抑制洛氏拟杆菌PK1295与其两种革兰氏阳性伙伴血链球菌34和以色列放线菌PK14之间共聚集的单克隆抗体，被用于对洛氏拟杆菌表面两种不同类型的菌毛相关黏附素进行计数和定位。用放射性标记单克隆抗体进行的结合研究表明，细胞表面存在最大数量（根据Scatchard图计算得出的Bmax）约400个对血链球菌具有特异性的黏附素分子以及310个对以色列放线菌具有特异性的黏附素分子。免疫电子显微镜显示，黏附素并非菌毛亚基的组成部分；相反，它们通常以随机方式分布在结构的远端部分。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d79d/210881/7fd0be0a56ee/jbacter00181-0110-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d79d/210881/7813bb45991d/jbacter00181-0108-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d79d/210881/f2d6b99d66b0/jbacter00181-0109-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d79d/210881/82c29ccc6168/jbacter00181-0110-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d79d/210881/7fd0be0a56ee/jbacter00181-0110-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d79d/210881/7813bb45991d/jbacter00181-0108-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d79d/210881/f2d6b99d66b0/jbacter00181-0109-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d79d/210881/82c29ccc6168/jbacter00181-0110-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d79d/210881/7fd0be0a56ee/jbacter00181-0110-b.jpg

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Host-specific fimbrial adhesins of noninvasive enterotoxigenic Escherichia coli strains.非侵袭性产肠毒素大肠杆菌菌株的宿主特异性菌毛黏附素。
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