de Ree J M, Schwillens P, van den Bosch J F
Department of Medical Microbiology, University of Limburg, Maastricht, The Netherlands.
Microb Pathog. 1987 Feb;2(2):113-21. doi: 10.1016/0882-4010(87)90103-3.
Pap fimbriae were purified from a recombinant strain and used for the production of monoclonal antibodies (MAbs). These MAbs were screened in a fimbriae ELISA with eight different P fimbriae as well as 1A and 1C fimbriae. Five MAbs were specific for Pap fimbriae whereas one MAb did react with Pap, F7(2) and F11 fimbriae. Previously, we described two F11 MAbs which also reacted with Pap, F7(2) and F11 fimbriae. In a whole bacteria ELISA it was shown that the MAbs, which recognized Pap, F7(2) and F11 fimbriae, reacted with recombinant strains which did not express Pap or F11 fimbriae, but still expressed the globoside binding properties. Not one of the five MAbs which are specific for Pap fimbriae reacted with these globoside binding recombinant strains. In a haemagglutination and adherence assay it was shown that only the MAbs which recognized the Pap, F7(2) and F11 fimbriae inhibited the adhesive properties of the globoside binding recombinant strain. Therefore it is concluded that in the present study MAbs are presented which recognize the minor components responsible for adhesion.
从重组菌株中纯化出P菌毛，用于制备单克隆抗体（MAb）。这些单克隆抗体在菌毛酶联免疫吸附测定（ELISA）中，用8种不同的P菌毛以及1A和1C菌毛进行筛选。5种单克隆抗体对P菌毛具有特异性，而1种单克隆抗体确实能与P菌毛、F7(2)菌毛和F11菌毛发生反应。此前，我们描述了两种也能与P菌毛、F7(2)菌毛和F11菌毛发生反应的F11单克隆抗体。在全菌ELISA中显示，识别P菌毛、F7(2)菌毛和F11菌毛的单克隆抗体，能与不表达P菌毛或F11菌毛但仍具有球苷结合特性的重组菌株发生反应。5种对P菌毛具有特异性的单克隆抗体中，没有一种能与这些具有球苷结合特性的重组菌株发生反应。在血凝和黏附试验中显示，只有识别P菌毛、F7(2)菌毛和F11菌毛的单克隆抗体能抑制具有球苷结合特性的重组菌株的黏附特性。因此得出结论，在本研究中展示的单克隆抗体识别负责黏附的次要成分。
