Department of Chemical Engineering and Materials Science, Stevens Institute of Technology, 1 Castle Point on Hudson, Hoboken, NJ, 07030, USA.
Department of Chemical Engineering and Materials Science, Stevens Institute of Technology, 1 Castle Point on Hudson, Hoboken, NJ, 07030, USA.
Colloids Surf B Biointerfaces. 2017 Dec 1;160:215-219. doi: 10.1016/j.colsurfb.2017.09.040. Epub 2017 Sep 18.
Conformational structures of lysozyme at the interfaces of hydrophilic polymer poly[2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl)ammonium hydroxide] (PMEDSAH), are examined to understand the role of protein-polymer interactions on the stability of lysozyme. This work underpins the effect of hydration layer on the structures of physically adsorbed lysozyme on PMEDSAH brushes. Hydrophilic nature and strength of hydration layers around brushes are controlled by varying the brush thickness and temperature. We measured that lysozyme is structurally less stable on 15nm thick hydrophilic PMEDSAH brushes at 75°C than at room temperature. To the contrary, 5-8nm thick brushes stretch in hydrated state by heating, hence yield higher structural stability of lysozyme. These results suggest that short polyzwitterionic brushes can facilitate improved biomaterial interactions that are essential for biosensors performing at elevated temperatures.
研究溶菌酶在亲水性聚合物聚[2-（甲基丙烯酰氧基）乙基二甲基-（3-磺丙基）铵氢氧化物]（PMEDSAH）界面的构象结构，以了解蛋白质-聚合物相互作用对溶菌酶稳定性的作用。这项工作的基础是水合层对物理吸附在 PMEDSAH 刷上的溶菌酶结构的影响。通过改变刷的厚度和温度来控制刷周围水合层的亲水性和强度。我们测量到，在 75°C 时，15nm 厚的亲水性 PMEDSAH 刷上的溶菌酶结构稳定性比在室温下差。相反，在加热时，5-8nm 厚的刷在水合状态下伸展，从而产生更高的溶菌酶结构稳定性。这些结果表明，短的聚两性离子刷可以促进改善生物材料相互作用，这对于在高温下运行的生物传感器至关重要。
