Department of Chemical & Biomolecular Engineering, National University of Singapore, Kent Ridge, Singapore, 119260, Singapore.
Biofouling. 2012;28(9):895-912. doi: 10.1080/08927014.2012.719895.
Dense and uniform polymer brush coatings were developed to combat marine biofouling. Nonionic hydrophilic, nonionic hydrophobic, cationic, anionic and zwitterionic polymer brush coatings were synthesized via surface-initiated atom transfer radical polymerization (SI-ATRP) of 2-hydroxyethyl methacrylate, 2,3,4,5,6-pentafluorostyrene, 2-(methacryloyloxy)ethyl trimethylammonium chloride, 4-styrenesulfonic acid sodium and N,N'-dimethyl-(methylmethacryloyl ethyl) ammonium propanesulfonate, respectively. The functionalized surfaces had different efficacies in preventing adsorption of bovine serum albumin (BSA), adhesion of the Gram-negative bacterium Pseudomonas sp. NCIMB 2021 and the Gram-positive Staphylococcus aureus, and settlement of cyprids of the barnacle Amphibalanus amphitrite (=Balanus amphitrite). The nonionic hydrophilic, anionic and zwitterionic polymer brushes resisted BSA adsorption during a 2 h exposure period. The nonionic hydrophilic, cationic and zwitterionic brushes exhibited resistance to bacterial fouling (24 h exposure) and cyprid settlement (24 and 48 h incubation). The hydrophobic brushes moderately reduced protein adsorption, and bacteria and cyprid settlement. The anionic brushes were least effective in preventing attachment of bacteria and barnacle cyprids. Thus, the best approach to combat biofouling involves a combination of nonionic hydrophilic and zwitterionic polymer brush coatings on material surfaces.
为了对抗海洋生物附着，制备了致密且均匀的聚合物刷涂层。通过 2-羟乙基甲基丙烯酸酯、2,3,4,5,6-五氟苯乙烯、2-（甲基丙烯酰氧基）乙基三甲基氯化铵、4-苯乙烯磺酸和 N,N'-二甲基-（甲基丙烯酰基乙基）铵丙磺酸盐的表面引发原子转移自由基聚合（SI-ATRP），分别合成了非离子型亲水性聚合物刷、非离子型疏水性聚合物刷、阳离子型聚合物刷、阴离子型聚合物刷和两性离子聚合物刷。功能化表面在防止牛血清白蛋白（BSA）吸附、革兰氏阴性菌假单胞菌 NCIMB 2021 和革兰氏阳性菌金黄色葡萄球菌黏附以及藤壶幼体附着方面具有不同的效果。非离子型亲水性、阴离子型和两性离子聚合物刷在 2 小时暴露期间抵抗 BSA 吸附。非离子型亲水性、阳离子型和两性离子刷表现出对细菌污染（24 小时暴露）和藤壶幼体附着（24 和 48 小时孵育）的抵抗能力。疏水性刷适度减少蛋白质吸附、细菌和藤壶幼体附着。阴离子刷在防止细菌和藤壶幼体附着方面效果最差。因此，对抗生物附着的最佳方法涉及在材料表面结合使用非离子型亲水性和两性离子聚合物刷涂层。
