Giustarini Daniela, Galvagni Federico, Colombo Graziano, Dalle-Donne Isabella, Milzani Aldo, Aloisi Anna Maria, Rossi Ranieri
Department of Medicine, Surgery and Neurosciences, University of Siena, Via A. Moro 2, I-53100 Siena, Italy.
Department of Biotechnology, Chemistry and Pharmacy, University of Siena, Via A. Moro 2, I-53100 Siena, Italy.
Anal Biochem. 2017 Dec 1;538:38-41. doi: 10.1016/j.ab.2017.09.010. Epub 2017 Sep 20.
We have introduced protein thiolation index (PTI), i.e. the molar ratio of the sum of all low molecular mass thiols bound to plasma proteins to protein free cysteinyl residues, as a sensitive biomarker of oxidative stress. According to the original procedure its determination requires a rapid separation of plasma and a specific treatment of samples to stabilize thiols. Here we demonstrate that samples can be collected without use of any anticoagulant to prevent blood clotting and without any stabilization of thiols too. This simplification of the determination of PTI makes its analysis more feasible also in routine clinical laboratories.
我们引入了蛋白质硫醇化指数（PTI），即与血浆蛋白结合的所有低分子量硫醇总和与游离蛋白半胱氨酰残基的摩尔比，作为氧化应激的敏感生物标志物。根据原始方法，其测定需要快速分离血浆并对样品进行特定处理以稳定硫醇。在这里，我们证明可以在不使用任何抗凝剂来防止血液凝固且不进行任何硫醇稳定处理的情况下采集样品。PTI测定方法的这种简化使得其分析在常规临床实验室中也更可行。
