Departamento de Anestesiología, Hospital General Universitario Gregorio Marañón, Madrid, Spain.
Departamento de Farmacología y Toxicología, Facultad Medicina, Universidad Complutense de Madrid, Madrid, Spain.
PLoS One. 2019 May 8;14(5):e0216359. doi: 10.1371/journal.pone.0216359. eCollection 2019.
Left ventricular hypertrophy (LVH) has been associated with oxidative stress, although not with the protein thiolation index (PTI). This study explored the potential use of PTI as a biomarker of oxidative stress in patients with LVH.
We recruited 70 consecutive patients (n = 35 LVH and n = 35 non-LVH) based on an echocardiography study in our institution (left ventricular mass indexed to body surface area). Plasma levels of both S-thiolated protein and total thiols were measured as biomarkers of oxidative stress by spectrophotometry, and PTI was calculated as the molar ratio between S-thiolated proteins and the total thiol concentration.
Values for plasma S-thiolated proteins were higher in patients with LVH than in the control group (P = 0.01). There were no differences in total thiols between the LVH group and the control group. Finally, PTI was higher in patients with LVH than in the control group (P = 0.001). The area under the ROC curve was 0.75 (95% CI, 0.63-0.86; P<0.001), sensitivity was 70.6%, and specificity was 68.6%, thus suggesting that PTI could be used to screen for LVH. A multivariable logistic regression model showed a positive association (P = 0.02) between PTI and LVH (OR = 1.24 [95% CI, 1.03-1.49]) independently of gender (OR = 3.39 [95% CI, 0.60-18.91]), age (OR = 1.03 [95% CI, 0.96-1.10]), smoking (OR = 5.15 [95% CI, 0.51-51.44]), glucose (OR = 0.99 [95% CI, 0.97-1.01]), systolic arterial pressure (OR = 1.10 [CI 1.03-1.17]), diastolic arterial pressure (OR = 0.94 [CI 0.87-1.02]), dyslipidemia (OR = 1.46 [95% CI, 0.25-8.55]), estimated glomerular filtration rate (OR = 0.98 [95% CI, 0.96-1.01]), body mass index (OR = 1.03 [95% CI, 0.90-1.10]), and valvular and/or coronary disease (OR = 5.27 [95% CI, 1.02-27.21]).
The present study suggests that PTI could be a new biomarker of oxidative stress in patients with LVH.
左心室肥厚（LVH）与氧化应激有关，但与蛋白硫醇指数（PTI）无关。本研究旨在探讨 PTI 作为 LVH 患者氧化应激生物标志物的潜在应用。
我们根据我院的超声心动图研究（左心室质量与体表面积之比），连续招募了 70 名患者（LVH 组 35 名，非 LVH 组 35 名）。通过分光光度法测量血浆中 S-硫醇化蛋白和总硫醇的水平，作为氧化应激的生物标志物，并计算 S-硫醇化蛋白与总硫醇浓度的摩尔比作为 PTI。
LVH 组患者的血浆 S-硫醇化蛋白水平高于对照组（P = 0.01）。LVH 组和对照组之间的总硫醇水平没有差异。最后，LVH 组的 PTI 高于对照组（P = 0.001）。ROC 曲线下面积为 0.75（95%CI，0.63-0.86；P<0.001），敏感性为 70.6%，特异性为 68.6%，因此提示 PTI 可用于筛查 LVH。多变量逻辑回归模型显示 PTI 与 LVH 之间存在正相关（P = 0.02）（OR = 1.24 [95%CI，1.03-1.49]），独立于性别（OR = 3.39 [95%CI，0.60-18.91]）、年龄（OR = 1.03 [95%CI，0.96-1.10]）、吸烟（OR = 5.15 [95%CI，0.51-51.44]）、血糖（OR = 0.99 [95%CI，0.97-1.01]）、收缩压（OR = 1.10 [1.03-1.17]）、舒张压（OR = 0.94 [0.87-1.02]）、血脂异常（OR = 1.46 [95%CI，0.25-8.55]）、估计肾小球滤过率（OR = 0.98 [95%CI，0.96-1.01]）、体重指数（OR = 1.03 [95%CI，0.90-1.10]）和瓣膜或冠状动脉疾病（OR = 5.27 [95%CI，1.02-27.21]）。
本研究提示 PTI 可能是 LVH 患者氧化应激的新生物标志物。
