一种新型长链非编码 RNA linc00460 可被 CBP/P300 上调，促进食管鳞状细胞癌的发生。
A novel long noncoding RNA linc00460 up-regulated by CBP/P300 promotes carcinogenesis in esophageal squamous cell carcinoma.
机构信息
Department of Medical Genetics, College of Basic Medical Science, Third Military Medical University, Gaotanyan Street, Shapingba District, Chongqing, China.
Department of Cardiothoracic Surgery, Southwest Hospital, Third Military Medical University, Gaotanyan Street, Shapingba District, Chongqing, China.
出版信息
Biosci Rep. 2017 Oct 17;37(5). doi: 10.1042/BSR20171019. Print 2017 Oct 31.
Esophageal cancer is one of the leading causes of cancer-related mortality because of poor prognosis. Long noncoding RNAs (lncRNAs) have been gradually demonstrated to play critical roles in cancer development. We identified a novel long noncoding RNA named linc00460 by microarray analysis using esophageal squamous cell carcinoma (ESCC) clinical samples, which has not been studied before. Our research indicated that linc00460 was overexpressed in the majority of tumor tissues and ESCC cell lines. Linc00460 expression was positively correlated with ESCC TNM stage, lymph node metastasis, and predicted poor prognosis. experiments showed that linc00460 depletion suppressed ESCC cell growth through regulating cell proliferation and cell cycle; in additional, linc00460 depletion accelerated ESCC cell apoptosis. We further revealed that linc00460 overexpression was manipulated by transcriptional co-activator CBP/P300 through histone acetylation. Given the high expression and important biological functions of linc00460, we suggest that linc00460 works as an oncogene and might be a valuable prognostic biomarker for ESCC diagnosis and treatment.
食管癌是癌症相关死亡的主要原因之一，因为预后不良。长链非编码 RNA（lncRNA）已逐渐被证明在癌症发展中发挥关键作用。我们通过使用食管鳞状细胞癌（ESCC）临床样本的微阵列分析鉴定了一种新型长链非编码 RNA，命名为 linc00460，以前尚未对此进行研究。我们的研究表明，linc00460 在大多数肿瘤组织和 ESCC 细胞系中过表达。linc00460 的表达与 ESCC 的 TNM 分期、淋巴结转移呈正相关，并预测预后不良。实验表明，linc00460 的耗竭通过调节细胞增殖和细胞周期抑制 ESCC 细胞生长；此外，linc00460 的耗竭加速了 ESCC 细胞凋亡。我们进一步揭示，转录共激活因子 CBP/P300 通过组蛋白乙酰化来操纵 linc00460 的过表达。鉴于 linc00460 的高表达和重要的生物学功能，我们认为 linc00460 是一种癌基因，可能是 ESCC 诊断和治疗有价值的预后生物标志物。
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