Li Y Z, Hu X D, Lai X M, Li Y F, Lei Y
Department of Burns and Plastic Surgery, Deyang People's Hospital, Deyang, China.
Clin Exp Dermatol. 2018 Jan;43(1):11-18. doi: 10.1111/ced.13225. Epub 2017 Sep 21.
Development of drug therapies and other techniques for wound care have resulted in significant improvement of the cure rate and shortening of the healing time for wounds. A modified technique of regulated oxygen-enriched negative pressure-assisted wound therapy (RO-NPT) has been reported.
To evaluate the efficacy and impact of RO-NPT on wound recovery and inflammation.
Infected wounds were established on 40 adult female white rabbits, which were then randomized to one of four groups: O group, regulated negative pressure-assisted wound therapy (RNPT) group, regulated oxygen-enriched negative pressure-assisted wound therapy (RO-NPT) group and healthy control (HC) group. Each day, the O group was treated with a constant oxygen supply (1 L/min) to the wound, while the RNPT group was treated with continuous regulated negative pressure (70 ± 5 mmHg) and the RNPT + O group was treated with both. The HC group was treated with gauze dressing alone, which was changed every day. Leucocyte count, colony count and wound-healing rate were calculated. Levels of tumour necrosis factor (TNF)-α, interleukin (IL)-1β and IL-8 were evaluated by ELISA.
RO-RNPT significantly decreased bacterial count and TNF-α level, and increased the wound-healing rate. IL-1β, IL-8 and leucocyte count had a tendency to increase in the early phase of inflammation and a tendency to decrease in the later phase of inflammation in the RO-RNPT group.
RO-NPT therapy assisted wound recovery and inflammation control compared with the RNPT and oxygen-enriched therapies. RO-NPT therapy also increased levels of IL-1β and IL-8 and attenuated expression of TNF-α in the early phase of inflammation.
药物治疗及其他伤口护理技术的发展已使伤口治愈率显著提高，愈合时间缩短。已有报道一种改良的富氧负压辅助伤口治疗（RO-NPT）技术。
评估RO-NPT对伤口愈合及炎症的疗效和影响。
在40只成年雌性白兔身上制造感染伤口，然后将其随机分为四组：O组、负压辅助伤口治疗（RNPT）组、富氧负压辅助伤口治疗（RO-NPT）组和健康对照组（HC）。每天，O组对伤口持续供氧（1升/分钟），RNPT组进行持续负压调节（70±5毫米汞柱），RO-NPT组同时进行这两种治疗。HC组仅用纱布敷料治疗，每天更换。计算白细胞计数、菌落计数和伤口愈合率。通过酶联免疫吸附测定法评估肿瘤坏死因子（TNF）-α、白细胞介素（IL）-1β和IL-8的水平。
RO-RNPT显著降低了细菌计数和TNF-α水平，并提高了伤口愈合率。在RO-RNPT组中，IL-1β、IL-8和白细胞计数在炎症早期有升高趋势，在炎症后期有降低趋势。
与RNPT和富氧疗法相比，RO-NPT疗法有助于伤口愈合和炎症控制。RO-NPT疗法还提高了IL-1β和IL-8水平，并在炎症早期减弱了TNF-α的表达。
