Faculty of Engineering & the Institute of Nanotechnology & Advanced Materials, Bar-Ilan University, Ramat-Gan, Israel.
Department of Internal Medicine, Meir Medical Center, Kfar Saba, Israel.
Nanomedicine (Lond). 2018 Aug 1;13(15):1835-1945. doi: 10.2217/nnm-2018-0053. Epub 2018 Aug 28.
Regulated negative pressure-assisted wound therapy is a fundamental, nonpharmaceutical technology for acute and chronically infected wounds, yet bacterial clearance and biofilm buildup remain a challenge for healing. Regulated irrigation combined with negative pressure (RI-NPT) is emerging as an alternative therapeutic strategy for reducing bacterial load. Here, we analyzed RI-NPT hydrokinetics and efficacy of bacterial load reduction in wounds.
MATERIALS & METHODS: Escherichia coli were loaded with gold nanoparticles, quantified by flame atomic absorption spectroscopy. Computed tomography (CT) imaging tracked bacterial distribution over time in a low-flow rat wound model. Bacterial load was quantified using a novel CT ruler.
Flame atomic absorption spectroscopy showed loading of 1.7 × 10 ± 0.2 gold nanoparticles/cell. CT tracking revealed that while regulated negative pressure-assisted wound therapy reduced bacterial load to a limited extent (5%), RI-NPT significantly increased bacterial outflow and clearance (by 45%).
This nanotechnology-based approach demonstrates that RI-NPT is essential for reducing bacterial load and, thus, for promoting wound healing.
调节负压辅助伤口治疗是治疗急性和慢性感染性伤口的基本非药物技术，但清除细菌和生物膜的形成仍然是愈合的一个挑战。调节冲洗结合负压（RI-NPT）作为一种替代治疗策略，正在出现以减少细菌负荷。在这里，我们分析了 RI-NPT 的流体动力学和减少伤口细菌负荷的效果。
用金纳米粒子负载大肠杆菌，通过火焰原子吸收光谱法进行定量。计算机断层扫描（CT）成像跟踪低流量大鼠伤口模型中细菌随时间的分布。使用新型 CT 标尺定量细菌负荷。
火焰原子吸收光谱法显示每个细胞负载 1.7×10±0.2 个金纳米粒子。CT 追踪显示，虽然调节负压辅助伤口治疗在一定程度上（5%）降低了细菌负荷，但 RI-NPT 显著增加了细菌的流出和清除（增加了 45%）。
这项基于纳米技术的方法表明，RI-NPT 对于减少细菌负荷，从而促进伤口愈合是至关重要的。
