This study was undertaken to evaluate whether exosomes from human adipose-derived stem cells (ASC-exo) can stimulate the regeneration of human dermal fibroblasts (HDFs). Immunoblotting and FACS analyses showed that ASC-exo was positive for exosome markers. Fluorescence tracking revealed that the contents of ASC-exo were transferred into the HDFs. ASC-exo treatment also stimulated the proliferation and migration of HDFs in a dose-dependent manner. Similarly, the expression levels of genes involved in skin cell proliferation were increased by ASC-exo. Microarray analysis showed an enrichment of microRNAs that have regenerative function. We suggest that the ASC-exo can stimulate skin cell proliferation.