Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan, P. R. China.
State Key Laboratory of Biocontrol and School of Life Sciences, Sun Yat-sen University, Guangzhou, P. R. China.
Proteomics. 2017 Nov;17(21). doi: 10.1002/pmic.201700165. Epub 2017 Oct 20.
Chitin synthases are very important enzymes for chitin synthesis in various species, which makes them a specific target of insecticides. In the present study, the function of the chitin synthase A (CHSA) gene isolated from Mythimna separate is investigated. The majority of dsMysCHSA treated larvae (89.50%) exhibit lethal phenotypes, including three phenotypes with severe cuticle deformations. The dsMysCHSA treatment in adult females affects oogenesis, and significantly reduce the ovary size and the oviposition number compared with controls. To determine how MysCHSA affects female fecundity, combined analyses of RNA-sequencing (RNA-Seq) transcriptome and TMT proteome (tandem mass tags) data in M. separata after treatment with MysCHSA-RNAi is performed. The differentially expressed proteins and genes affect fecundity-related proteins, energy metabolism, fatty acid metabolism, amino sugars, and nucleotide sugar metabolism pathways. Taken together, these results suggest that MysCHSA acts on M. separata ecdysis and fecundity, and has the potential as a target gene for pest control.
几丁质合酶对于各种生物的几丁质合成非常重要，这使得它们成为杀虫剂的特定靶标。在本研究中，研究了从粘虫中分离出的几丁质合酶 A (CHSA)基因的功能。大多数经 dsMysCHSA 处理的幼虫（89.50%）表现出致命表型，包括三种严重的表皮畸形表型。dsMysCHSA 处理对成年雌性的卵发生有影响，与对照组相比，卵巢大小和产卵数量显著减少。为了确定 MysCHSA 如何影响雌性生殖力，对 MysCHSA-RNAi 处理后的粘虫进行了 RNA 测序 (RNA-Seq) 转录组和 TMT 蛋白质组（串联质量标签）数据的联合分析。差异表达的蛋白质和基因影响与生殖力相关的蛋白质、能量代谢、脂肪酸代谢、氨基糖和核苷酸糖代谢途径。综上所述，这些结果表明 MysCHSA 作用于粘虫蜕皮和生殖力，具有作为害虫防治靶标基因的潜力。
