College of Life and Health Science, Kaili University, Kaili 556011, China.
Provincial Key Laboratory for Agricultural Pest Management of Mountainous Regions, Institute of Entomology, Guizhou University, Guiyang 550025, China.
Genes (Basel). 2022 Jul 27;13(8):1340. doi: 10.3390/genes13081340.
UDP--acetylglucosamine pyrophosphorylase (UAP) is a key enzyme in the chitin biosynthesis pathway of insects. Here, we described the gene in the white-backed planthopper (Horváth) with an open reading frame of 1470 bp. Quantitative real-time polymerase chain reaction (qPCR) suggested that exhibits a different developmental expression pattern and a higher expression after molting. The highest expression of was observed in the integument tissues of adults, whereas head tissues showed negligible expression. RNAi-based gene silencing decreased the mRNA transcript levels in nymphs injected with double-stranded RNA of . Finally, silencing led to 84% mortality and malformed phenotypes in nymphs. Thus, our results can help better understand the role of in .
UDP--N-乙酰氨基葡萄糖焦磷酸化酶（UAP）是昆虫几丁质生物合成途径中的一种关键酶。在这里，我们描述了白背飞虱（Horváth）中的基因，其开放阅读框为 1470bp。定量实时聚合酶链反应（qPCR）表明，表现出不同的发育表达模式，蜕皮后表达水平更高。在成虫的表皮组织中观察到的表达最高，而头部组织的表达可以忽略不计。基于 RNAi 的基因沉默会降低注射双链 RNA 的若虫中的 mRNA 转录本水平。最后，沉默导致 84%的若虫死亡和畸形表型。因此，我们的研究结果有助于更好地理解在白背飞虱中的作用。
