State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, China; Department of Traditional Chinese medicine, Zhejiang A&F University, Hangzhou 311300, China.
State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, China; Department of Traditional Chinese medicine, Zhejiang A&F University, Hangzhou 311300, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Feb 5;190:283-289. doi: 10.1016/j.saa.2017.09.030. Epub 2017 Sep 13.
Saffron is an expensive spice. Its primary effective constituents are crocin I and II, and the contents of these compounds directly affect the quality and commercial value of saffron. In this study, near-infrared spectroscopy was combined with chemometric techniques for the determination of crocin I and II in saffron. Partial least squares regression models were built for the quantification of crocin I and II. By comparing different spectral ranges and spectral pretreatment methods (no pretreatment, vector normalization, subtract a straight line, multiplicative scatter correction, minimum-maximum normalization, eliminate the constant offset, first derivative, and second derivative), optimum models were developed. The root mean square error of cross-validation values of the best partial least squares models for crocin I and II were 1.40 and 0.30, respectively. The coefficients of determination for crocin I and II were 93.40 and 96.30, respectively. These results show that near-infrared spectroscopy can be combined with chemometric techniques to determine the contents of crocin I and II in saffron quickly and efficiently.
藏红花是一种昂贵的香料。其主要有效成分是西红花苷 I 和 II，这些化合物的含量直接影响藏红花的质量和商业价值。本研究采用近红外光谱结合化学计量学技术测定藏红花中的西红花苷 I 和 II。建立了偏最小二乘回归模型对西红花苷 I 和 II 进行定量分析。通过比较不同的光谱范围和光谱预处理方法（无预处理、矢量归一化、减去一条直线、乘法散射校正、最大-最小归一化、消除常数偏移、一阶导数和二阶导数），开发了最佳模型。西红花苷 I 和 II 的最佳偏最小二乘模型的交叉验证值的均方根误差分别为 1.40 和 0.30，西红花苷 I 和 II 的决定系数分别为 93.40 和 96.30。这些结果表明，近红外光谱可以与化学计量学技术相结合，快速有效地测定藏红花中西红花苷 I 和 II 的含量。
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