Said-Fernández S, López-Revilla R
Unidad de Investigación Biomédica del Noreste (Instituto Mexicano del Seguro Social), Monterrey NL.
Infect Immun. 1988 Apr;56(4):874-9. doi: 10.1128/iai.56.4.874-879.1988.
The major hemolytic activity of Entamoeba histolytica trophozoites is located in a vesicular fraction called P30 and known to be due to heat-labile and heat-stable hemolytic components whose effect increases up to 100 times during preincubation at 36 degrees C. The heat-stable hemolytic activity (HSHA) was found in the chloroform-methanol extract of preincubated P30, whose partition with 2 M KCl yielded a lipid fraction, an interphase, and an aqueous phase. HSHA was detected only in the lipid fraction, where it amounted to 59% of the chloroform-methanol extract activity and increased 50% when supplemented with the interphase material; it was accounted for by the free fatty acids, whose potency increased 33% with the interphase material, and was blocked by delipidated bovine serum albumin. A parallel increase in free fatty acids and lysophospholipids and a corresponding decrease in phospholipids were observed during P30 preincubation. Most of the phospholipase activity of trophozoite homogenates was also found in P30. Therefore, most of the HSHA generated during preincubation was due to free fatty acids released from phospholipids by a P30 phospholipase that may contribute significantly to E. histolytica cytopathogenicity and virulence.
溶组织内阿米巴滋养体的主要溶血活性位于一个称为P30的囊泡部分，已知这是由于热不稳定和热稳定的溶血成分所致，其作用在36℃预孵育期间可增加至100倍。在预孵育的P30的氯仿 - 甲醇提取物中发现了热稳定溶血活性（HSHA），其与2M KCl的分配产生了一个脂质部分、一个界面相和一个水相。HSHA仅在脂质部分中检测到，其占氯仿 - 甲醇提取物活性的59%，当补充界面相物质时增加50%；它由游离脂肪酸引起，游离脂肪酸的效力随界面相物质增加33%，并被脱脂牛血清白蛋白阻断。在P30预孵育期间观察到游离脂肪酸和溶血磷脂平行增加，磷脂相应减少。滋养体匀浆的大部分磷脂酶活性也存在于P30中。因此，预孵育期间产生的大部分HSHA是由于P30磷脂酶从磷脂中释放的游离脂肪酸，这可能对溶组织内阿米巴的细胞致病性和毒力有显著贡献。
