Said-Fernández S, López-Revilla R
Z Parasitenkd. 1983;69(4):435-8. doi: 10.1007/BF00927699.
We have found that the hemolytic potency of P30 - a subcellular fraction that contains most of the hemolytic activity of Entamoeba histolytica trophozoites--increased up to 100 times by preincubation for 36 h at 36 degrees C. This remarkable increase in potency was due to the in vitro generation of heat-labile as well as heat-stable hemolytic components and could be prevented if P30 was preincubated at 4 degrees C or subjected to either heating at 90 degrees C or to repeated freeze-thawing cycles before preincubation at 36 degrees C. The major hemolytic activity of E. histolytica thus appears to depend on latent, heat-labile and heat-stable hemolysins that are probably unmasked through the action of endogenous amebal enzymes.
我们发现，P30（一种包含溶组织内阿米巴滋养体大部分溶血活性的亚细胞组分）在36℃预孵育36小时后，其溶血能力提高了100倍。这种显著的能力增强是由于体外产生了热不稳定和热稳定的溶血成分，如果P30在4℃预孵育，或者在36℃预孵育前先在90℃加热或进行反复冻融循环，这种增强就可以被阻止。因此，溶组织内阿米巴的主要溶血活性似乎取决于潜在的、热不稳定和热稳定的溶血素，这些溶血素可能通过内源性阿米巴酶的作用而被暴露出来。
