Hackenmueller Sarah A, Scanlan Thomas S
Department of Physiology and Pharmacology, Oregon Health and Science University, Portland, Oregon, USA.
Synth Commun. 2013;43(10):1439-1446. doi: 10.1080/00397911.2011.639005. Epub 2012 Jun 5.
Thyroid hormones undergo extensive metabolism to regulate hormone activity. A labeled thyroid hormone would be useful to track hormone metabolism through various pathways. While radiolabeled thyroid hormones have been synthesized and used for in vivo studies, a stable isotope labeled form of thyroid hormone is required for studying thyroid hormone metabolism by LC-MS/MS, an analytical technique that has certain advantages without the complications of radioactivity. Here we report the synthesis of C-N-T and C-N-T, two labeled thyroid hormone derivatives suitable for in vivo LC-MS/MS studies.
甲状腺激素经历广泛的代谢以调节激素活性。一种标记的甲状腺激素将有助于追踪激素通过各种途径的代谢。虽然放射性标记的甲状腺激素已被合成并用于体内研究,但通过液相色谱-串联质谱法(LC-MS/MS)研究甲状腺激素代谢需要一种稳定同位素标记形式的甲状腺激素,这是一种具有某些优势且无放射性并发症的分析技术。在此,我们报告了两种适用于体内LC-MS/MS研究的标记甲状腺激素衍生物C-N-T和C-N-T的合成。