Murray G I, Burke M D, Ewen S W
Department of Pathology, University of Aberdeen, Foresterhill, UK.
Histochem J. 1987 Sep;19(9):476-82. doi: 10.1007/BF01675417.
A method is described for the histochemical demonstration of gamma-glutamyl transpeptidase in tissue sections embedded in glycol methacrylate at low temperature. Enzyme activity was preserved by a short (3 h) fixation of tissue in 4% paraformaldehyde at 4 degrees C prior to embedding at 4 degrees C. Tissue embedded in glycol methacrylate combined good morphology with accurate enzyme localization. Blocks of the embedded tissue could be stored at room temperature for at least 3 months without loss of enzyme activity. The resin is non-fluorescent, allowing the use of the fluorescent coupling agent 5-nitrosalicylaldehyde to visualize the reaction product.
本文描述了一种在低温下对包埋于乙二醇甲基丙烯酸酯中的组织切片进行γ-谷氨酰转肽酶组织化学显示的方法。在4℃包埋前，将组织在4℃用4%多聚甲醛短时间（3小时）固定，可保留酶活性。包埋于乙二醇甲基丙烯酸酯中的组织兼具良好的形态和准确的酶定位。包埋组织块可在室温下保存至少3个月而酶活性不丧失。该树脂无荧光，允许使用荧光偶联剂5-亚硝基水杨醛来观察反应产物。
