作为用于蛋白质分析研究的化学探针的炔烃取代色氨酸的合成。
synthesis of alkyne substituted tryptophans as chemical probes for protein profiling studies.
作者信息
Nair R N, Rosnow J J, Murphree T A, Bowden M E, Lindemann S R, Wright A T
机构信息
Biological Sciences Division, Pacific Northwest National Laboratory, Richland. WA.
R. N. Nair. Current address - David Geffen School of Medicine, Department of Psychiatry and Behavorial Sciences, University of California, Los Angeles.
出版信息
Org Chem Front. 2017 Apr 1;4(4):495-499. doi: 10.1039/C6QO00819D. Epub 2016 Dec 26.
Abstract
synthesis of alkynalted tryptophan moieties as chemical probes for protein profilling studies, via an unexpected synthesis of Michael acceptor 12 is reported. Friedel Craft's alkylation of 12 and alkyne substituted indoles gave alkynalated tryptophan moieties, which perform as chemical probe by incorporating into actively translating cells, whereby the alkyne moiety enables multimodal analyses through click chemistry mediated attachment of reporting groups.
摘要
报道了通过意外合成迈克尔受体12来合成炔基化色氨酸部分作为蛋白质分析研究的化学探针。12与炔基取代的吲哚进行傅克烷基化反应得到炔基化色氨酸部分，其通过掺入活跃翻译的细胞中作为化学探针，其中炔基部分通过点击化学介导的报告基团连接实现多模态分析。
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