Cheng H, Pittman K A, Dandekar K A
Metabolism and Pharmacokinetics Department, Bristol-Myers Company, Buffalo, NY 14213.
J Pharm Sci. 1987 Dec;76(12):918-9. doi: 10.1002/jps.2600761216.
A simple and sensitive assay for quantitating 9-methyl-3-(1H-tetrazol-5-yl)-4H-pyrido[1,2-a]pyrimidin-4-one (1; BMY 26517) in human plasma was developed using high-performance liquid chromatography with fluorescence detection. The method involves precipitation of protein and reversed-phase chromatography. The method is linear in the range of 4.3-429 ng/mL of 1, and the limit of detection is 0.4 ng/mL. The day-to-day precision values of this method at 25.7 and 386 ng/mL are 2.1 and 2.6%, respectively. The day-to-day accuracy values at these concentrations are 99.7 and 99.8%, respectively. The recovery of 1 is 98.3%.
建立了一种简单灵敏的高效液相色谱荧光检测法，用于定量测定人血浆中的9-甲基-3-(1H-四氮唑-5-基)-4H-吡啶并[1,2-a]嘧啶-4-酮（1；BMY 26517）。该方法包括蛋白质沉淀和反相色谱法。该方法在1的浓度范围为4.3 - 429 ng/mL时呈线性，检测限为0.4 ng/mL。该方法在25.7和386 ng/mL时的日间精密度值分别为2.1%和2.6%。这些浓度下的日间准确度值分别为99.7%和99.8%。1的回收率为98.3%。
