2型腺病毒L1 RNA 3'末端在体内和体外形成的分析
Analysis of adenovirus type 2 L1 RNA 3'-end formation in vivo and in vitro.
作者信息
Hales K H, Birk J M, Imperiale M J
机构信息
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109-0620.
出版信息
J Virol. 1988 Apr;62(4):1464-8. doi: 10.1128/JVI.62.4.1464-1468.1988.
Abstract
Downstream sequence requirements for efficient cleavage and polyadenylation at the adenovirus type 2 L1 poly(A) site were determined in vivo in 293 cells and in vitro by using RNA precursors in HeLa cell nuclear extracts. The two cleavage sites used were found to differ in sensitivity to 3'-end deletion in vivo and in vitro.
摘要
通过在293细胞中进行体内实验以及在体外利用HeLa细胞核提取物中的RNA前体，确定了腺病毒2型L1聚腺苷酸化位点高效切割和聚腺苷酸化的下游序列要求。发现在体内和体外，所使用的两个切割位点对3'端缺失的敏感性不同。
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