Department of Mechanical Engineering, Graduate School, Kyungpook National University, Daegu 41566, Republic of Korea.
Biomed Mater. 2017 Sep 25;12(5):055010. doi: 10.1088/1748-605X/aa7b51.
Ligament, a fibrous connective tissue between bones, is a unique tissue in human anatomy because it has complex viscoelastic properties and is very tough. Moreover, it is an important tissue for regeneration because frequent injuries occur, but there are limited types of substitutes that can be used as a tissue replacement. In this study, we present a stem cell-laden fiber/hydrogel composite structure with a layered fibrous structure, which can enhance cell infiltration, topographical cue and mechanical properties. It can promote cell viability, proliferation, and differentiation of the ligament phenotype with the help of a growth factor. The mechanical properties of the developed structure were experimentally identified using tensile tests, while cell viability and various functionalities were verified through culture tests using mesenchymal stem cells.
韧带是一种连接骨的纤维结缔组织，它在人体解剖学中是一种独特的组织，因为它具有复杂的黏弹性和非常坚韧的特性。此外，由于经常受伤，韧带是一种重要的再生组织，但可作为组织替代物的类型有限。在这项研究中，我们提出了一种具有分层纤维结构的干细胞负载纤维/水凝胶复合材料结构，这种结构可以增强细胞浸润、形貌线索和机械性能。在生长因子的帮助下，它可以促进细胞活力、增殖和韧带表型的分化。通过拉伸试验实验性地确定了所开发结构的机械性能，而通过使用间充质干细胞进行培养试验则验证了细胞活力和各种功能。
