[药用植物的DNA标记辅助选择（Ⅰ）.三七抗病品种的选育研究]

[DNA marker-assisted selection of medicinal plants (Ⅰ) .Breeding research of disease-resistant cultivars of Panax notoginseng].

作者信息

Liu Min, Huang Wen-Li, Wang Xiao, Wan Rui-Han, Wang Hai, Yan Zhu-Yun

机构信息

The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine, Key Laboratory of Systematic Research, Development and Utilization of Chinese Medicine Resources in Sichuan Province-Key Laboratory Breeding Base of Co-founded by Sichuan Province and Ministry of Science and Technology, Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2017 Jan;42(1):70-75. doi: 10.19540/j.cnki.cjcmm.20161222.039.

DOI:10.19540/j.cnki.cjcmm.20161222.039

PMID:28945027

Abstract

The study is aimed to estimate the diversity of arbuscular mycorrhizal fungi (AMF) in the main producing areas of Salvia miltiorrhiza.Diversity of AMF was surveyed directly on spores isolated from the field soil, collected from 20 sites of 8 provinces. Identification of the AMF was made by observation of spore morphology. At least 27 recognized AMF species were identified in samples from field soil, belonging to seven genera of AMF-Acaulospora, Glomus, Funneliformis, Ambispora, Rhizophagus, Pacispora, and Claroideoglomus. Acaulospora and Glomus were the dominant genera, respectively including nine and eight species. A. laevis (90%), R.manihotis (80%), A. brieticulata (75%), A. tuberculata (70%) were the dominant species.Colonization rate was determined,colonization was easily found, but the colonization intensities were low, the colonization rate remained at 10.92%-25.93%. The similarity between provinces is generally low, and the similarity coefficients were from 0.20 to 0.57.

摘要

本研究旨在评估丹参主要产区丛枝菌根真菌（AMF）的多样性。通过直接观察从8个省份20个地点采集的田间土壤中分离出的孢子，对AMF的多样性进行了调查。通过观察孢子形态对AMF进行鉴定。在田间土壤样本中至少鉴定出27种已确认的AMF物种，分属于AMF的7个属——无梗囊霉属、球囊霉属、漏斗孢球囊霉属、双孢无梗囊霉属、根内球囊霉属、类球囊霉属和明球囊霉属。无梗囊霉属和球囊霉属为优势属，分别包含9个和8个物种。平滑无梗囊霉（90%）、木薯根内球囊霉（80%）、短网无梗囊霉（75%）、瘤状无梗囊霉（70%）为优势种。测定了定殖率，定殖现象容易发现，但定殖强度较低，定殖率维持在10.92%-25.93%。省份之间的相似性普遍较低，相似系数为0.20至0.57。