Huang Wen-li, Fan Xin-jian, Yan Zhu-yun, Ma Yun-tong, Meng Xian-li
College of Pharmacy, Chengdu University of TCM, Key Lab of Standardization of Chinese Herbal Medicines of Ministry of Education, Chengdu 610075, China.
Zhong Yao Cai. 2012 May;35(5):689-93.
To investigate the resources and distribution of Arbuscular Mycorrhizal Fungi (AMF) in rhizosphere of Coptis deltoidea.
The colonization situation of AMF of Coptis deltoidea were detected by acid fuchsin staining and then calculated the colonization rate. AMF fungal spores were obtained by Wet-screening method and then classified and identified by their morphological characteristics and histochemical staining method.
Coptis deltoidea and AMF could engender the mycorrhiza. Colonization rates were different in different producing areas and ranged from 23. 3% to 34.4%. Thirty species of AMF belonging in 6 genera were found, including 17 species of the Acaulospora, 7 of the Glomus, 2 of the Gigaspora, 1 of the Entrophospora, 1 of the Paraglomus and 2 of the Archaeospora were isolated from rhizosphere of Coptis deltoidea. Acaulospora and Glomus were the dominant genera and the AMF species of genuine producing regions in Hongya county was the most diversiform among the six areas.
Coptis deltoidea is a kind of nutrition type of mycorrhiza plant. The rich diversity of AMF of Coptis deltoidea in producing areas make the AMF is a biological resource of significant application prospects in enlarging yield and non-pollution production.
研究三角叶黄连根际丛枝菌根真菌（AMF）资源及其分布情况。
采用酸性品红染色法检测三角叶黄连AMF的侵染状况，并计算侵染率。通过湿筛法获得AMF真菌孢子，然后依据其形态特征和组织化学染色法进行分类鉴定。
三角叶黄连与AMF可形成菌根。不同产地的侵染率有所差异，范围在23.3%至34.4%之间。从三角叶黄连根际分离出隶属于6个属的30种AMF，其中无梗囊霉属17种、球囊霉属7种、巨孢囊霉属2种、内养囊霉属1种、类球囊霉属1种、古孢囊霉属2种。无梗囊霉属和球囊霉属为优势属，洪雅县道地产区的AMF种类在6个地区中最为多样。
三角叶黄连是一种菌根营养型植物。三角叶黄连产区丰富多样的AMF是扩大产量和进行无污染生产具有重要应用前景的生物资源。
