壳聚糖包覆氧化铁纳米颗粒的抗菌和抗真菌活性

Antibacterial and antifungal activity of chitosan coated iron oxide nanoparticles.

作者信息

Nehra P, Chauhan R P, Garg N, Verma K

机构信息

a School of Biomedical Engineering , National Institute of Technology , Kurukshetra , India.

b Department of Physics , National Institute of Technology , Kurukshetra , India.

出版信息

Br J Biomed Sci. 2018 Jan;75(1):13-18. doi: 10.1080/09674845.2017.1347362. Epub 2017 Sep 25.

DOI:10.1080/09674845.2017.1347362

PMID:28945174

Abstract

BACKGROUND AND AIM

The emergence of resistance against antimicrobial agents has led to the development of more efficient agents and new techniques for treatment of various microbial infections. The aim of the present study is to determine the antibacterial and antifungal activity of bare and chitosan coated FeO nanoparticles (NPs) against five organisms, Escherichia coli (E. coli), Bacillus subtilis (B. subtilis), Candida albicans (C. albicans), Aspergillus niger (A. niger) and Fusarium solani (F. solani).

METHODS

FeO NPs were synthesised by coprecipitation and surface coating was done by chitosan polymer to avoid agglomeration. The antimicrobial property of NPs was tested by agar well diffusion and analysed by measuring the diameter of the inhibition zone.

RESULTS

Average particle size of FeO and chitosan coated FeO NPs was 10.4 ± 4.9 and 11.4 ± 5.2 nm, respectively. Mean diameter of inhibition zone of synthesised chitosan coated FeO NPs was in the range 14.5 to 18.5 mm. The effect of chitosan coated Iron oxide nanoparticles was F. solani/A. niger < C. albicans < E. coli/B. subtilis (p < 0.001).

CONCLUSIONS

Chitosan coated FeO NPs are effective antimicrobial agents and so may be developed as a microbial resistant coating for biomedical devices.

摘要

背景与目的

对抗菌药物耐药性的出现促使人们研发更有效的药物以及治疗各种微生物感染的新技术。本研究旨在测定裸露的和壳聚糖包覆的FeO纳米颗粒（NPs）对五种微生物的抗菌和抗真菌活性，这五种微生物分别是大肠杆菌（E. coli）、枯草芽孢杆菌（B. subtilis）、白色念珠菌（C. albicans）、黑曲霉（A. niger）和茄病镰刀菌（F. solani）。

方法

通过共沉淀法合成FeO NPs，并使用壳聚糖聚合物进行表面包覆以避免团聚。通过琼脂孔扩散法测试NPs的抗菌性能，并通过测量抑菌圈直径进行分析。

结果

FeO和壳聚糖包覆的FeO NPs的平均粒径分别为10.4 ± 4.9和11.4 ± 5.2 nm。合成的壳聚糖包覆的FeO NPs的抑菌圈平均直径在14.5至18.5 mm范围内。壳聚糖包覆的氧化铁纳米颗粒的抑菌效果为茄病镰刀菌/黑曲霉 < 白色念珠菌 < 大肠杆菌/枯草芽孢杆菌（p < 0.001）。