Venkateswarulu M, Gambhir Diksha, Kaur Harpreet, Daniel P Vineeth, Mondal Prosenjit, Koner Rik Rani
School of Basic Sciences, Indian Institute of Technology Mandi, Mandi-175001, H.P., India.
Dalton Trans. 2017 Oct 3;46(38):13118-13125. doi: 10.1039/c7dt02387a.
We demonstrate a strategy for the recognition of As in aqueous solution using a red-emissive probe based on a perylene-Cu ensemble decorated with peripheral free carboxyl functionality. Single crystal analysis helped us to understand the chemical structure of the probe. To the best of our knowledge, this is the first probe for arsenic detection which emits in the red region (λ = 600 nm). The perylene-Cu ensemble exhibited a mega-Stokes shift (>100 nm) with a high degree of selectivity upon interaction with As, which indicated that the present probe has the potential to be used as a turn-on optical sensor for selective detection of As with fewer experimental limitations. The detection limit was found to be 26 nM. Inspired by its good emissive properties, the ensemble was further explored for imaging As in live cells. Because of its long-range emissive nature, no autofluorescence from the cellular species was observed during the imaging process. The probe was evaluated to be non-toxic and successfully permeated the cell membrane without the help of any permeabilizing agent to image As.
我们展示了一种使用基于带有外围游离羧基官能团的苝 - 铜配合物的红色发射探针识别水溶液中砷的策略。单晶分析帮助我们了解了该探针的化学结构。据我们所知，这是首个用于砷检测且在红色区域（λ = 600 nm）发射的探针。苝 - 铜配合物与砷相互作用时表现出大斯托克斯位移（>100 nm）和高度选择性，这表明该探针有潜力用作开启型光学传感器，用于选择性检测砷，且实验限制较少。检测限为26 nM。受其良好发光特性的启发，该配合物进一步用于活细胞中砷的成像研究。由于其长程发光性质，成像过程中未观察到细胞物质的自发荧光。该探针经评估无毒，无需任何通透剂的帮助就能成功穿透细胞膜对砷进行成像。
