DeHaan Hunter, McCambridge Aidan, Armstrong Brittany, Cruse Carlie, Solanki Dhruv, Trinidad Jonathan C, Arkov Alexey L, Gao Ming
Biology Department, Indiana University Northwest, Gary, IN, USA.
Department of Chemistry, Indiana University, Bloomington, IN, USA.
FEBS Lett. 2017 Nov;591(21):3536-3547. doi: 10.1002/1873-3468.12854. Epub 2017 Oct 11.
Drosophila Me31B is a conserved protein of germ granules, ribonucleoprotein complexes essential for germ cell development. Me31B post-transcriptionally regulates mRNAs by interacting with other germ granule proteins. However, a Me31B interactome is lacking. Here, we use an in vivo proteomics approach to show that the Me31B interactome contains polypeptides from four functional groups: RNA regulatory proteins, glycolytic enzymes, cytoskeleton/motor proteins, and germ plasm components. We further show that Me31B likely colocalizes with the germ plasm components Tudor (Tud), Vasa, and Aubergine in the nuage and germ plasm and provide evidence that Me31B may directly bind to Tud in a symmetrically dimethylated arginine-dependent manner. Our study supports the role of Me31B in RNA regulation and suggests its novel roles in germ granule assembly and function.
果蝇Me31B是生殖颗粒中的一种保守蛋白，生殖颗粒是核糖核蛋白复合物，对生殖细胞发育至关重要。Me31B通过与其他生殖颗粒蛋白相互作用，在转录后水平调节mRNA。然而，目前还缺乏Me31B相互作用组。在这里，我们使用体内蛋白质组学方法表明，Me31B相互作用组包含来自四个功能组的多肽：RNA调节蛋白、糖酵解酶、细胞骨架/运动蛋白和生殖质成分。我们进一步表明，Me31B可能与生殖质成分都铎蛋白（Tud）、Vasa和茄子蛋白在生殖质和生殖颗粒中共定位，并提供证据表明Me31B可能以对称二甲基化精氨酸依赖的方式直接与Tud结合。我们的研究支持了Me31B在RNA调节中的作用，并表明其在生殖颗粒组装和功能中的新作用。
