莫氏锥度锥形连接假体连接处的微生物渗漏：一项体外研究。

Microbial Leakage at Morse Taper Conometric Prosthetic Connection: An In Vitro Investigation.

机构信息

*Associate Professor, Department of Prosthetic Dentistry, School of Dentistry, University of Padova, Padova, Italy. †PhD Student, Department of Oral and Maxillofacial Surgery and Oral Medicine, Faculty of Odontology, University of Malmö, Malmö, Sweden. ‡Associate Professor, Department of Oral and Maxillofacial Surgery and Oral Medicine, Faculty of Odontology, University of Malmö, Malmö, Sweden. §Laboratory Analyst, Biomolecular Diagnostic S.r.l., Firenze, Italy. ¶Laboratory Director, Biomolecular Diagnostic S.r.l., Firenze, Italy. ‖Associate Professor, Department of Periodontology, Institute of Odontology, The Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden.[LINE SEPARATOR] #Assistant Professor, Department of Prosthodontics, Dental Clinic, School of Dentistry, University of Milan, Milan, Italy.

出版信息

Implant Dent. 2017 Oct;26(5):756-761. doi: 10.1097/ID.0000000000000657.

DOI:10.1097/ID.0000000000000657

PMID:28945671

Abstract

OBJECTIVE

To evaluate in vitro the sealing capability at the prosthetic connection interface of 2 conometric systems.

MATERIALS AND METHODS

Two conometric systems with the same design and different material were used, for a total of 24 samples. Each sample was assembled by a tapered abutment and respective coping. In group A, the copings were made of gold, whereas in group B they were made of PEEK. Three μL of mix bacterial suspension (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and Fusobacterium nucleatum species) was inoculated into the abutment screw hole, and the coping was inserted on the abutment. Samples were immersed into culture tubes and incubated for 24, 48, and 72 hours into anaerobic conditions. Visual evaluation of turbidity was performed at each time point. Qualitative-quantitative assessment using real-time polymerase chain reaction was performed at 72 hours. Any difference between the groups was checked by means of Fisher exact test.

RESULTS

Microbial leakage occurred in both groups, and there was no statistically significant difference between groups. Microbial concentration resulted in a presence inferior to 1 × 10 copies/μL in all positive assemblies.

CONCLUSIONS

Because of the low bacterial count, it can be concluded that a minimal bacterial infiltration may be allowed by conometric interfaces for prosthetic connection.

摘要

目的

评估 2 种锥度连接系统在修复体连接界面的密封性能。

材料与方法

使用了 2 种设计相同但材料不同的锥度连接系统，共 24 个样本。每个样本由一个锥形基台和相应的修复体组成。在 A 组中，修复体由金制成，而在 B 组中，修复体由 PEEK 制成。将 3μL 混合细菌悬浮液（伴放线放线杆菌、牙龈卟啉单胞菌和核梭杆菌）接种到基台螺丝孔中，然后将修复体插入基台上。将样本浸入培养管中，在厌氧条件下孵育 24、48 和 72 小时。在每个时间点进行浊度的视觉评估。在 72 小时时进行实时聚合酶链反应的定性定量评估。通过 Fisher 精确检验检查组间的任何差异。