Department of Head and Neck Surgery and Maxillofacial Surgery, Heliópolis Hospital, São Paulo, Brazil.
Clin Oral Implants Res. 2010 Mar;21(3):328-35. doi: 10.1111/j.1600-0501.2009.01837.x. Epub 2010 Jan 13.
The aim of this study was to determine and compare the frequency of bacterial leakage of Streptococcus sanguinis biotype II along the implant-abutment interface between two systems of morse taper dental implants. Different methods of activation of the taper abutments were used: tapped-in (Bicon) and screwed-in (Ankylos).
Twenty sterile assemblies were used and attached, 10 Bicon and 10 Ankylos implants, according to manufacturers' specifications. They were then totally immersed within 20 test tubes containing a sterile nutrient solution brain-heart infusion (BHI). The internal part of the 20 implants was previously inoculated with 0.1 microl of S. sanguinis II (ATCC 10557) and then connected to the respective abutments. The assemblies were incubated under anaerobic conditions for 14 days in an autoclave at 37 degrees C. They were monitored daily for solution cloudiness resultant from microbial leakage on the interface of the assemblies. For statistical analysis, the Fisher test was applied and significance was assigned at the 5% level.
There was solution cloudiness, indicating the finding of bacterial growth inside two Bicon assemblies and two Ankylos assemblies 48 h after incubation. Microbial leakage was further substantiated by testing the suspension for the presence of Streptococcus sp. None of the sterility controls were contaminated. The frequency of bacterial leakage along the implant-abutment interface, with the two different morse taper implant systems, was 20% of the assemblies of each system. There were no statistical differences between them.
Irrespective of which of the two morse taper implant connection systems of activation was analyzed, tapped-in (Bicon) or screwed-in (Ankylos), this in vitro experiment showed bacterial leakage along the implant-abutment interface.
本研究旨在确定并比较两种莫氏锥度种植体系统中，沿种植体-基台界面的血链球菌生物型 II 的细菌渗漏频率。使用了两种不同的锥度基台激活方法：旋入式（Bicon）和旋入式（Ankylos）。
使用了 20 个无菌组件，并按照制造商的规格分别连接 10 个 Bicon 和 10 个 Ankylos 种植体。然后，将它们完全浸入 20 个装有无菌脑心浸液（BHI）的试管中。20 个种植体的内部部分事先接种了 0.1 微升的 S. sanguinis II（ATCC 10557），然后与相应的基台连接。将组件在 37°C 的高压灭菌器中进行厌氧孵育 14 天。每天监测组件界面是否有微生物渗漏导致溶液混浊。为了进行统计分析，应用了 Fisher 检验，并在 5%的水平上分配了显著性。
孵育 48 小时后，两个 Bicon 组件和两个 Ankylos 组件中有溶液混浊，表明内部有细菌生长。通过测试悬浮液中是否存在链球菌 sp. 进一步证实了微生物渗漏。所有的无菌对照都没有被污染。两种不同莫氏锥度种植体系统的种植体-基台界面细菌渗漏的频率均为每个系统组件的 20%。它们之间没有统计学差异。
无论分析的是哪种莫氏锥度种植体连接系统的激活方式，旋入式（Bicon）还是旋入式（Ankylos），本体外实验均显示细菌沿种植体-基台界面渗漏。
