Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310058, China.
Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310058, China; Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, China.
Sci Total Environ. 2018 Feb 1;613-614:783-791. doi: 10.1016/j.scitotenv.2017.09.157. Epub 2017 Sep 21.
The wide application of an organophosphate pesticide triazophos raises concern on the environmental pollution and the potential risk to human health. Thus, it is crucial to regularly monitor triazophos residue in the environment and agro-products. Herein we described a non-competitive immunoassay for trace detection of triazophos using a direct surface plasmon resonance (SPR) biosensor. Two anti-triazophos monoclonal antibodies (mAbs) were immobilized on the sensor chip and characterized by SPR-based kinetic analysis. The mAb with relatively slow dissociation rate was used for direct immunosensing of triazophos. The biosensor assay showed a high specificity and a low detection limit of 0.096ngmL to triazophos, with the linear detection range of 0.98-8.29ngmL. Under the optimal condition, the sensor chip could be regenerated for 160cycles at least. Moreover, the sensitive method was applied to determine triazophos in the spiked environmental water and agricultural products, as well as in unknown real-life samples (including Chinese cabbage, cucumber, and apple). Desirable results demonstrated that the newly-developed immunosensor could be used as a rapid, convenient, and reliable tool to regularly monitor triazophos and meet the detection requirement of its maximum residue limits.
有机磷农药三唑磷的广泛应用引起了人们对环境污染和对人类健康潜在风险的关注。因此，定期监测环境和农产品中的三唑磷残留量至关重要。本文描述了一种使用直接表面等离子体共振（SPR）生物传感器痕量检测三唑磷的非竞争免疫分析方法。两种抗三唑磷单克隆抗体（mAb）被固定在传感器芯片上，并通过基于 SPR 的动力学分析进行了表征。具有相对较慢解离速率的 mAb 用于三唑磷的直接免疫检测。生物传感器分析显示出对三唑磷的高特异性和低检测限为 0.096ngmL，线性检测范围为 0.98-8.29ngmL。在最佳条件下，传感器芯片至少可以再生 160 个循环。此外，该灵敏方法还用于测定环境水样和农产品中添加的三唑磷，以及未知的实际生活样品（包括白菜、黄瓜和苹果）中的三唑磷。令人满意的结果表明，新开发的免疫传感器可作为一种快速、方便、可靠的工具，用于定期监测三唑磷，并满足其最大残留限量的检测要求。
