Center for RNA Research, Institute for Basic Science, and School of Biological Sciences, Seoul National University, Seoul 08826, Korea.
Center for RNA Research, Institute for Basic Science, School of Biological Sciences, and Bioinformatics Institute, Seoul National University, Seoul 08826, Korea.
BMB Rep. 2017 Nov;50(11):554-559. doi: 10.5483/bmbrep.2017.50.11.179.
MicroRNAs (miRNAs) are ∼22nt-long single-stranded RNA molecules that form a RNA-induced silencing complex with Argonaute (AGO) protein to post-transcriptionally downregulate their target messenger RNAs (mRNAs). To understand the regulatory mechanisms of miRNA, discovering the underlying functional rules for how miRNAs recognize and repress their target mRNAs is of utmost importance. To determine functional miRNA targeting rules, previous studies extensively utilized various methods including high-throughput biochemical assays and bioinformatics analyses. However, targeting rules reported in one study often fail to be reproduced in other studies and therefore the general rules for functional miRNA targeting remain elusive. In this review, we evaluate previously-reported miRNA targeting rules and discuss the biological impact of the functional miRNAs on gene-regulatory networks as well as the future direction of miRNA targeting research. [BMB Reports 2017; 50(11): 554-559].
微小 RNA（miRNA）是约 22nt 长的单链 RNA 分子，与 Argonaute（AGO）蛋白形成 RNA 诱导的沉默复合物，从而对其靶信使 RNA（mRNA）进行转录后下调。为了了解 miRNA 的调控机制，发现 miRNA 识别和抑制其靶 mRNA 的潜在功能规则至关重要。为了确定功能 miRNA 靶向规则，以前的研究广泛利用了包括高通量生化测定和生物信息学分析在内的各种方法。然而，一项研究中报告的靶向规则往往不能在其他研究中重现，因此功能 miRNA 靶向的一般规则仍难以捉摸。在这篇综述中，我们评估了以前报道的 miRNA 靶向规则，并讨论了功能 miRNA 对基因调控网络的生物学影响，以及 miRNA 靶向研究的未来方向。[BMB 报告 2017；50(11):554-559]。
