Guangdong Key Laboratory of Laboratory Animals, Guangzhou 510633, China.
Guangdong Laboratory Animals Monitoring Institute, Guangzhou 510633, China.
J Virol Methods. 2017 Dec;250:41-46. doi: 10.1016/j.jviromet.2017.09.022. Epub 2017 Sep 23.
Murine parvovirus is one of the most prevalent infectious pathogens in mouse colonies. A specific primer pair targeting the VP2 gene of minute virus of mice (MVM) and mouse parvovirus (MPV) was utilized for high resolution melting (HRM) analysis. The resulting melting curves could distinguish these two virus strains and there was no detectable amplification of the other mouse pathogens which included rat parvovirus (KRV), ectromelia virus (ECT), mouse adenovirus (MAD), mouse cytomegalovirus (MCMV), polyoma virus (Poly), Helicobactor hepaticus (H. hepaticus) and Salmonella typhimurium (S. typhimurium). The detection limit of the standard was 10 copies/μL. This study showed that the PCR-HRM assay could be an alternative useful method with high specificity and sensitivity for differentiating murine parvovirus strains MVM and MPV.
鼠细小病毒是鼠群中最常见的传染性病原体之一。本研究使用针对微小鼠病毒（MVM）和鼠细小病毒（MPV）VP2 基因的特异性引物对进行高分辨率熔解（HRM）分析。所得的熔解曲线可区分这两种病毒株，并且其他鼠病原体（包括大鼠细小病毒（KRV）、疱疹病毒（ECT）、鼠腺病毒（MAD）、鼠巨细胞病毒（MCMV）、多瘤病毒（Poly）、鼠伤寒沙门氏菌（H. hepaticus）和鼠伤寒沙门氏菌（S. typhimurium））均无可检测到的扩增。标准的检测限为 10 拷贝/μL。本研究表明，PCR-HRM 检测法具有高度特异性和敏感性，可作为区分 MVM 和 MPV 鼠细小病毒株的一种替代有用方法。
